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Insulin secretion f...
Insulin secretion from beta-cells is affected by deletion of nicotinamide nucleotide transhydrogenase.
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Shimomura, Kenju (author)
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Galvanovskis, Juris (author)
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Goldsworthy, Michelle (author)
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Hugill, Alison (author)
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Kaizak, Stephan (author)
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Lee, Angela (author)
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Meadows, Nicholas (author)
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Quwailid, Mohamed Mohideen (author)
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- Rydström, Jan, 1943 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry
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Teboul, Lydia (author)
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Ashcroft, Fran (author)
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Cox, Roger D (author)
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(creator_code:org_t)
- 2009
- 2009
- English.
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In: Methods in enzymology. - 1557-7988. ; 457, s. 451-80
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Abstract
Subject headings
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- Nicotinamide nucleotide transhydrogenase (NNT) is an inner mitochondrial membrane transmembrane protein involved in regenerating NADPH, coupled with proton translocation across the inner membrane. We have shown that a defect in Nnt function in the mouse, and specifically within the beta-cell, leads to a reduction in insulin secretion. This chapter describes methods for examining Nnt function in the mouse. This includes generating in vivo models with point mutations and expression of Nnt by transgenesis, and making in vitro models, by silencing of gene expression. In addition, techniques are described to measure insulin secretion, calcium and hydrogen peroxide concentrations, membrane potential, and NNT activity. These approaches and techniques can also be applied to other genes of interest.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Animals
- Calcium
- analysis
- Cell Line
- Gene Silencing
- Hydrogen Peroxide
- analysis
- Insulin
- secretion
- Insulin-Secreting Cells
- enzymology
- secretion
- Membrane Potential
- Mitochondrial
- Mice
- Mice
- Inbred C3H
- Mice
- Inbred C57BL
- Mice
- Transgenic
- Microscopy
- Confocal
- Mitochondria
- enzymology
- genetics
- NADP Transhydrogenase
- genetics
- metabolism
- Point Mutation
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Shimomura, Kenju
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Galvanovskis, Ju ...
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Goldsworthy, Mic ...
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Hugill, Alison
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Kaizak, Stephan
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Lee, Angela
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show more...
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Meadows, Nichola ...
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Quwailid, Mohame ...
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Rydström, Jan, 1 ...
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Teboul, Lydia
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Ashcroft, Fran
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Cox, Roger D
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Methods in enzym ...
- By the university
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University of Gothenburg