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A microfluidic devi...
A microfluidic device for reversible environmental changes around single cells using optical tweezers for cell selection and positioning
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- Eriksson, Emma, 1980 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg
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- Sott, Kristin, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg
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- Lundqvist, Fredrik, 1979 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi, mikrobiologi,Institutionen för fysik (GU),Department of Cell and Molecular Biology, Microbiology,Department of Physics (GU),University of Gothenburg
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- Sveningsson, Martin, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg
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Scrimgeour, Jan, 1980 (author)
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- Hanstorp, Dag, 1960 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg
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- Goksör, Mattias, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg
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- Graneli, Annette, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg
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(creator_code:org_t)
- 2010
- 2010
- English.
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In: Lab Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 10:5, s. 617-625
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Abstract
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- Cells naturally exist in a dynamic chemical environment, and therefore it is necessary to study cell behaviour under dynamic stimulation conditions in order to understand the signalling transduction pathways regulating the cellular response. However, until recently, experiments looking at the cellular response to chemical stimuli have mainly been performed by adding a stress substance to a population of cells and thus only varying the magnitude of the stress. In this paper we demonstrate an experimental method enabling acquisition of data on the behaviour of single cells upon reversible environmental perturbations, where microfluidics is combined with optical tweezers and fluorescence microscopy. The cells are individually selected and positioned in the measurement region on the bottom surface of the microfluidic device using optical tweezers. The optical tweezers thus enable precise control of the cell density as well as the total number of cells within the measurement region. Consequently, the number of cells in each experiment can be optimized while clusters of cells, that render subsequent image analysis more difficult, can be avoided. The microfluidic device is modelled and demonstrated to enable reliable changes between two different media in less than 2 s. The experimental method is tested by following the cycling of GFP-tagged proteins (Mig1 and Msn2, respectively) between the cytosol and the nucleus in Saccharomyces cerevisiae upon changes in glucose availability.
Subject headings
- NATURVETENSKAP -- Fysik -- Den kondenserade materiens fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Condensed Matter Physics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
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Lab Chip
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Eriksson, Emma, ...
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Sott, Kristin, 1 ...
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Lundqvist, Fredr ...
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Sveningsson, Mar ...
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Scrimgeour, Jan, ...
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Hanstorp, Dag, 1 ...
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Goksör, Mattias, ...
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Graneli, Annette ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Condensed Matter ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Other Physics To ...
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Lab Chip
- By the university
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University of Gothenburg
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Chalmers University of Technology