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Micropipet-assisted...
Micropipet-assisted formation of microscopic networks of unilamellar lipid bilayer nanotubes and containers
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- Karlsson, Mattias, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Sott, Kristin, 1974 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Cans, Ann-Sofie, 1971 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Karlsson, Anders (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Karlsson, Roger, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Orwar, Owe, 1964 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2001-10-06
- 2001
- English.
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In: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 17:22, s. 6754-6758
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http://dx.doi.org/10...
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Abstract
Subject headings
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- We describe a novel micropipet-assisted technique for the construction of complex, surface-immobilized two-dimensional microscopic networks of unilamellar phospholipid bilayer vesicles (1-50 pm in diameter, 10(-15)-10(-12) L) interconnected by lipid nanotubes (100-300 nm in diameter). As starting material for the construction of networks, we used twinned vesicle pairs, one of which is multilamellar and functions as a membrane donor and the other unilamellar and functions as a membrane acceptor upon manipulation. By electromechanical insertion of a pipet tip into the unilamellar vesicle followed by lateral pulling of the micropipet away from the vesicle, a nanotube was formed. Buffer solution contained in the pipet was then injected into the nanotube orifice, forming a vesicle of controlled size that was immobilized on the surface. The networks have controlled connectivity and are well-defined with regard to the container size, angle between nanotube extensions, and nanotube length. The internal fluid composition of individual vesicles is defined during the formation of a network by selection of the solution contained in the micropipet.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- PHOSPHOLIPID-VESICLES
- LIVING CELLS
- PROTEIN
- MOLECULES
- LIPOSOMES
- TUBULES
- FUSION
- LIPOSOMES
Publication and Content Type
- ref (subject category)
- art (subject category)
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Langmuir
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- By the author/editor
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Karlsson, Mattia ...
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Sott, Kristin, 1 ...
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Cans, Ann-Sofie, ...
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Karlsson, Anders
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Karlsson, Roger, ...
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Orwar, Owe, 1964
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Langmuir
- By the university
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University of Gothenburg
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Chalmers University of Technology