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Guidelines for the ...
Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes.
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Galluzzi, L (author)
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Aaronson, S A (author)
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Abrams, J (author)
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show more...
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Alnemri, E S (author)
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Andrews, D W (author)
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Baehrecke, E H (author)
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Bazan, N G (author)
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Blagosklonny, M V (author)
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- Blomgren, Klas, 1963 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institutionen för kliniska vetenskaper,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation,Institute of Clinical Sciences
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Borner, C (author)
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Bredesen, D E (author)
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Brenner, C (author)
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Castedo, M (author)
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Cidlowski, J A (author)
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Ciechanover, A (author)
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Cohen, G M (author)
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De Laurenzi, V (author)
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De Maria, R (author)
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Deshmukh, M (author)
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Dynlacht, B D (author)
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El-Deiry, W S (author)
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Flavell, R A (author)
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Fulda, S (author)
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Garrido, C (author)
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Golstein, P (author)
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Gougeon, M-L (author)
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Green, D R (author)
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Gronemeyer, H (author)
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Hajnóczky, G (author)
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Hardwick, J M (author)
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Hengartner, M O (author)
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Ichijo, H (author)
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Jäättelä, M (author)
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Kepp, O (author)
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Kimchi, A (author)
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Klionsky, D J (author)
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Knight, R A (author)
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Kornbluth, S (author)
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Kumar, S (author)
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Levine, B (author)
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Lipton, S A (author)
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Lugli, E (author)
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Madeo, F (author)
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Malomi, W (author)
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Marine, J-C W (author)
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Martin, S J (author)
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Medema, J P (author)
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Mehlen, P (author)
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Melino, G (author)
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Moll, U M (author)
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Morselli, E (author)
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Nagata, S (author)
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Nicholson, D W (author)
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Nicotera, P (author)
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Nuñez, G (author)
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Oren, M (author)
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Penninger, J (author)
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Pervaiz, S (author)
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Peter, M E (author)
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Piacentini, M (author)
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Prehn, J H M (author)
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Puthalakath, H (author)
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Rabinovich, G A (author)
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Rizzuto, R (author)
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Rodrigues, C M P (author)
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Rubinsztein, D C (author)
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Rudel, T (author)
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Scorrano, L (author)
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Simon, H-U (author)
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Steller, H (author)
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Tschopp, J (author)
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Tsujimoto, Y (author)
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Vandenabeele, P (author)
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Vitale, I (author)
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Vousden, K H (author)
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Youle, R J (author)
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Yuan, J (author)
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- Zhivotovsky, B (author)
- Karolinska Institutet
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Kroemer, G (author)
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(creator_code:org_t)
- 2009-04-17
- 2009
- English.
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In: Cell death and differentiation. - : Springer Science and Business Media LLC. - 1476-5403 .- 1350-9047. ; 16:8, s. 1093-107
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https://www.nature.c...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Apoptosis
- Cell Death
- Eukaryotic Cells
- cytology
- Flow Cytometry
- Guidelines as Topic
- Humans
- Immunoblotting
- Microscopy
- Electron
- Scanning
- Microscopy
- Fluorescence
- Spectrometry
- Fluorescence
Publication and Content Type
- ref (subject category)
- for (subject category)
Find in a library
To the university's database
- By the author/editor
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Galluzzi, L
-
Aaronson, S A
-
Abrams, J
-
Alnemri, E S
-
Andrews, D W
-
Baehrecke, E H
-
show more...
-
Bazan, N G
-
Blagosklonny, M ...
-
Blomgren, Klas, ...
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Borner, C
-
Bredesen, D E
-
Brenner, C
-
Castedo, M
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Cidlowski, J A
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Ciechanover, A
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Cohen, G M
-
De Laurenzi, V
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De Maria, R
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Deshmukh, M
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Dynlacht, B D
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El-Deiry, W S
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Flavell, R A
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Fulda, S
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Garrido, C
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Golstein, P
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Gougeon, M-L
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Green, D R
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Gronemeyer, H
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Hajnóczky, G
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Hardwick, J M
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Hengartner, M O
-
Ichijo, H
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Jäättelä, M
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Kepp, O
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Kimchi, A
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Klionsky, D J
-
Knight, R A
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Kornbluth, S
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Kumar, S
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Levine, B
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Lipton, S A
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Lugli, E
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Madeo, F
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Malomi, W
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Marine, J-C W
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Martin, S J
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Medema, J P
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Mehlen, P
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Melino, G
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Moll, U M
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Morselli, E
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Nagata, S
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Nicholson, D W
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Nicotera, P
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Nuñez, G
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Oren, M
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Penninger, J
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Pervaiz, S
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Peter, M E
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Piacentini, M
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Prehn, J H M
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Puthalakath, H
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Rabinovich, G A
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Rizzuto, R
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Rodrigues, C M P
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Rubinsztein, D C
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Rudel, T
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Scorrano, L
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Simon, H-U
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Steller, H
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Tschopp, J
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Tsujimoto, Y
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Vandenabeele, P
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Vitale, I
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Vousden, K H
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Youle, R J
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Yuan, J
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Zhivotovsky, B
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Kroemer, G
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
- Articles in the publication
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Cell death and d ...
- By the university
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University of Gothenburg
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Karolinska Institutet