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Differential locomo...
Differential locomotion of long- and short-term IL-2-activated murine natural killer cells in a model matrix environment.
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- Albertsson, Per, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
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Basse, P H (author)
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- Edsparr, Karin, 1979 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
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Kim, M H (author)
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Goldfarb, R H (author)
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Kitson, R P (author)
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- Lennernäs, Bo, 1963 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences
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- Nannmark, Ulf, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Johansson, Bengt R, 1947 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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(creator_code:org_t)
- Wiley, 2007
- 2007
- English.
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In: Scandinavian journal of immunology. - : Wiley. - 0300-9475 .- 1365-3083. ; 66:4, s. 402-9
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Abstract
Subject headings
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- Tumour infiltration by activated natural killer (A-NK) cells is a pre-requisite for tumour eradication by adoptive NK cell transfer. Extravasated A-NK cells do not always succeed in reaching the crucial target cell conjugation. Therefore, we wished to study A-NK cell locomotion and interactions with melanoma cells in a matrix environment (Matrigel) by electron, confocal and fluorescence microscopy. Two distinct patterns of A-NK cell-mediated matrix disintegration were revealed during incubation of tumour cells and A-NK cells in Matrigel: (1) A-NK cells pre-cultured for 5 days altered the homogeneous texture of the Matrigel, an initial microporous appearance became a loose filamentous meshwork by 24 h. Matrix degrading protease inhibitors could not fully prevent this, but could delay the process; and (2) A-NK cells pre-cultured for 6 days or more, instead formed large excavations in the Matrigel leaving the remaining matrix less affected compared to the effects by the younger A-NK cells. By histochemical staining with Cupromeronic Blue, the excavations were shown to contain proteoglycan material. Protease inhibitors had no discernable effect on the development of the excavations. The conspicuous capacity of A-NK cells to disintegrate extracellular matrix and the formation of large excavations seems only partially to depend on matrix-degrading proteases. Formation of extracellular proteoglycan material is suggested to facilitate A-NK cell locomotion within a matrix environment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Animals
- Cell Line
- Tumor
- Cell Movement
- immunology
- Coculture Techniques
- Collagen
- Drug Combinations
- Histocytochemistry
- Humans
- Indoles
- chemistry
- Interleukin-2
- immunology
- Killer Cells
- Lymphokine-Activated
- immunology
- Laminin
- Male
- Melanoma
- Experimental
- immunology
- Mice
- Mice
- Inbred C57BL
- Microscopy
- Confocal
- Microscopy
- Electron
- Transmission
- Microscopy
- Fluorescence
- Organometallic Compounds
- chemistry
- Proteoglycans
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Albertsson, Per, ...
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Basse, P H
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Edsparr, Karin, ...
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Kim, M H
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Goldfarb, R H
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Kitson, R P
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show more...
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Lennernäs, Bo, 1 ...
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Nannmark, Ulf, 1 ...
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Johansson, Bengt ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Scandinavian jou ...
- By the university
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University of Gothenburg