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- Albertsson, Per,1964Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences (author)
Differential locomotion of long- and short-term IL-2-activated murine natural killer cells in a model matrix environment.
- Article/chapterEnglish2007
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- Wiley,2007
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- LIBRIS-ID:oai:gup.ub.gu.se/120974
- https://gup.ub.gu.se/publication/120974URI
- https://doi.org/10.1111/j.1365-3083.2007.01956.xDOI
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- Language:English
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- Tumour infiltration by activated natural killer (A-NK) cells is a pre-requisite for tumour eradication by adoptive NK cell transfer. Extravasated A-NK cells do not always succeed in reaching the crucial target cell conjugation. Therefore, we wished to study A-NK cell locomotion and interactions with melanoma cells in a matrix environment (Matrigel) by electron, confocal and fluorescence microscopy. Two distinct patterns of A-NK cell-mediated matrix disintegration were revealed during incubation of tumour cells and A-NK cells in Matrigel: (1) A-NK cells pre-cultured for 5 days altered the homogeneous texture of the Matrigel, an initial microporous appearance became a loose filamentous meshwork by 24 h. Matrix degrading protease inhibitors could not fully prevent this, but could delay the process; and (2) A-NK cells pre-cultured for 6 days or more, instead formed large excavations in the Matrigel leaving the remaining matrix less affected compared to the effects by the younger A-NK cells. By histochemical staining with Cupromeronic Blue, the excavations were shown to contain proteoglycan material. Protease inhibitors had no discernable effect on the development of the excavations. The conspicuous capacity of A-NK cells to disintegrate extracellular matrix and the formation of large excavations seems only partially to depend on matrix-degrading proteases. Formation of extracellular proteoglycan material is suggested to facilitate A-NK cell locomotion within a matrix environment.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- Animals
- Cell Line
- Tumor
- Cell Movement
- immunology
- Coculture Techniques
- Collagen
- Drug Combinations
- Histocytochemistry
- Humans
- Indoles
- chemistry
- Interleukin-2
- immunology
- Killer Cells
- Lymphokine-Activated
- immunology
- Laminin
- Male
- Melanoma
- Experimental
- immunology
- Mice
- Mice
- Inbred C57BL
- Microscopy
- Confocal
- Microscopy
- Electron
- Transmission
- Microscopy
- Fluorescence
- Organometallic Compounds
- chemistry
- Proteoglycans
Added entries (persons, corporate bodies, meetings, titles ...)
- Basse, P H (author)
- Edsparr, Karin,1979Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences(Swepub:gu)xedspk (author)
- Kim, M H (author)
- Goldfarb, R H (author)
- Kitson, R P (author)
- Lennernäs, Bo,1963Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper,Institute of Clinical Sciences(Swepub:gu)xlennb (author)
- Nannmark, Ulf,1958Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xnanul (author)
- Johansson, Bengt R,1947Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology(Swepub:gu)xjbenp (author)
- Göteborgs universitetInstitutionen för kliniska vetenskaper (creator_code:org_t)
Related titles
- In:Scandinavian journal of immunology: Wiley66:4, s. 402-90300-94751365-3083
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