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Epithelial regenera...
Epithelial regeneration from bioengineered skin explants in culture.
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Mirastschijski, U (författare)
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Bugdahl, R (författare)
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Rollman, O (författare)
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- Johansson, Bengt R, 1947 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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Agren, M S (författare)
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(creator_code:org_t)
- 2005-11-18
- 2006
- Engelska.
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Ingår i: The British journal of dermatology. - : Oxford University Press (OUP). - 0007-0963. ; 154:1, s. 42-9
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- BACKGROUND: Artificial skin substitutes are beneficial in the treatment of chronic wounds although their performance relative to authentic human skin is unclear. OBJECTIVES: We compared the rate of outgrowth and morphology of neoepidermis from a bioengineered skin construct (Apligraf) with normal adult human skin explants on de-epidermized human dermal growth substrate with or without intact epidermal basement membrane zone. METHODS: Epithelial outgrowth of air-exposed cultures in serum-supplemented keratinocyte medium was quantified by fluorescence imaging, morphology by light microscopy, biomarkers of keratinocyte activation, proliferation and migration by immunohistochemical analysis, and gelatinases by zymography. RESULTS: Resurfacing from bioengineereed skin explants started earlier than from normal skin but subsequently, from day 3 to day 9, the rate of epidermalization from bioengineered skin was only 40% (206 +/- 23 microm day(-1), mean +/- SEM) of that of authentic skin (521 +/- 17 microm day(-1), P < 0.001). At culture termination at day 11, normal human skin had formed a multilayered and well-structured neoepidermis covering 41.0 +/- 1.2 mm2 of the dermal substrate while bioengineered skin produced a thinner, less organized epithelium covering 20.4 +/- 3.0 mm2. At this later stage, a higher expression of beta-defensin-2, keratin 16, Ki67 and matrix metalloproteinase (MMP)-9 was found in neoepidermis formed from authentic skin than from bioengineered skin. Activated MMP-2 was elevated in bioengineered skin-derived neoepidermis. Minor epithelial outgrowth was noted with either skin type on the dermal substrate devoid of basement membrane zone. CONCLUSIONS: Cultured normal skin explants produced a more uniform and expansive in vivo-like neoepidermis than bioengineered skin explants.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Nyckelord
- Adult
- Basement Membrane
- physiology
- Collagen
- physiology
- Epidermis
- metabolism
- physiology
- ultrastructure
- Epithelium
- physiology
- Female
- Humans
- Immunoenzyme Techniques
- Keratin-16
- Keratinocytes
- physiology
- Keratins
- metabolism
- Matrix Metalloproteinase 2
- metabolism
- Matrix Metalloproteinase 9
- metabolism
- Microscopy
- Electron
- Scanning
- Regeneration
- Skin
- Artificial
- Tissue Culture Techniques
- Tissue Engineering
- methods
- beta-Defensins
- metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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