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Progesterone recept...
Progesterone receptor antagonists Org 31710 and RU 486 increase apoptosis in human periovulatory granulosa cells.
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- Svensson, Eva Ch (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi,Institute of Physiology and Pharmacology
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- Markström, Emilia (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute of Physiology and Pharmacology, Dept of Physiology
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- Shao, Linus Ruijin, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute of Physiology and Pharmacology, Dept of Physiology
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- Andersson, Madeleine (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för oftalmologi,Institute of Clinical Neurosciences, Section of Ophtalmology
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- Billig, Håkan, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi,Institute of Physiology and Pharmacology, Dept of Physiology
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(creator_code:org_t)
- 2001
- 2001
- English.
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In: Fertility and sterility. - 0015-0282. ; 76:6, s. 1225-31
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Abstract
Subject headings
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- OBJECTIVE: To investigate if progesterone receptor (PR)-mediated effects are involved in regulating the susceptibility to apoptosis in LH receptor-stimulated human luteinizing granulosa cells. DESIGN: Laboratory study. SETTING: Göteborg University and an in vitro fertilization laboratory of a university hospital. PATIENT(S): Women undergoing oocyte retrieval for in vitro fertilization after ovulation induction with gonadotropins. INTERVENTION(S): Luteinizing granulosa cells were isolated from follicular aspirates after oocyte removal. The cells were treated with or without RU 486 (1 microM-100 microM), Org 31710 (1 microM-100 microM), progesterone (1 nM-10 microM), dexamethasone (0.5 microM-100 microM), dihydrotestosterone (1 nM-25 microM), RU 486 (10 microM-100 microM) + dexamethasone (50 microM), and picrotoxin (1 microM-100 microM) and were cultured under serum-free conditions. MAIN OUTCOME MEASURE(S): Measurement of caspase-3 activity; detection of internucleosomal DNA fragmentation using gel electrophoresis and fluorospectrophotometry; progesterone analysis of spent medium. RESULT(S): Addition of the PR antagonists RU 486 or Org 31710 in vitro to human luteinizing granulosa cells caused an increase in caspase-3 activity and a dose-dependent increase in internucleosomal DNA fragmentation. No effect on DNA fragmentation was seen after addition of dexamethasone, dihydrotestosterone, or picrotoxin. CONCLUSION(S): Nuclear PR-mediated effects are involved in regulating the susceptibility to apoptosis in LH receptor-stimulated human luteinizing granulosa cells.
Keyword
- Caspase 3
- Caspases
- metabolism
- DNA Fragmentation
- drug effects
- Dexamethasone
- pharmacology
- Dihydrotestosterone
- pharmacology
- Electrophoresis
- Agar Gel
- Estrenes
- pharmacology
- Female
- Furans
- pharmacology
- GABA Antagonists
- pharmacology
- Glucocorticoids
- pharmacology
- Granulosa Cells
- cytology
- drug effects
- Hormone Antagonists
- pharmacology
- Humans
- Mifepristone
- pharmacology
- Nucleosomes
- drug effects
- metabolism
- Picrotoxin
- pharmacology
- Progesterone
- pharmacokinetics
- pharmacology
- Receptors
- GABA-A
- deficiency
- Receptors
- Progesterone
- antagonists & inhibitors
- Spectrometry
- Fluorescence
Publication and Content Type
- ref (subject category)
- art (subject category)
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