The establishment of 20 different human embryonic stem cell lines and subclones; a report on derivation, culture, characterisation and banking.

This report summarises our efforts in deriving, characterising and banking of 20 different human embryonic stem cell lines. We have derived a large number of human embryonic stem cell lines between 2001 and 2005. One of these cell lines was established under totally xeno-free culture conditions. In addition, several subclones have been established, including a karyoptypical normal clone from a trisomic mother line. A master cell banking system has been utilised in concert with an extensive characterisation programme, ensuring a supply of high quality pluripotent stem cells for further research and development. In this report we also present the first data on a proprietary novel antibody, hES-Cellect, that exhibits high specificity for undifferentiated hES cells. In addition to the traditional manual dissection approach of propagating hES cells, we here also report on the successful approaches of feeder-free cultures as well as single cell cultures based on enzymatic digestion. All culture systems used as reported here have maintained the hES cells in a karyotypical normal and pluripotent state. These systems also have the advantage of being the principal springboards for further scale up of cultures for industrial or clinical applications that would require vastly more cells that can be produced by mechanical means.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
Animals
Cell Culture Techniques
methods
Cell Differentiation
drug effects
Cell Line
Clone Cells
cytology
Collagen
pharmacology
Dissection
Drug Combinations
Embryonic Stem Cells
cytology
drug effects
metabolism
Humans
Immunohistochemistry
In Situ Hybridization
Fluorescence
Karyotyping
Laminin
pharmacology
Mice
Proteoglycans
pharmacology
Quality Control
Telomerase
metabolism
Tissue Banks
Cell banking
Characterisation
Pluripotency
Single cells
Feeder-free
Xeno-free
hES cells
Derivation

Caisander, Gunilla (author)
Noaksson, Karin (author)
Emanuelsson, Katarina (author)
Lundin, Kersti,1957Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology(Swepub:gu)xluker (author)
Bergh, Christina,1953Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology(Swepub:gu)xbechr (author)
Hanson, Charles,1958Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi,Institute of Clinical Sciences, Department of Obstetrics and Gynecology(Swepub:gu)xhchaq (author)
Semb, HenrikLund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)endo-hse (author)
Strehl, Raimund (author)
Hyllner, Johan (author)
Göteborgs universitetInstitutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi (creator_code:org_t)

In:In vitro cellular & developmental biology. Animal: Springer Science and Business Media LLC46:3-4, s. 217-301543-706X1071-2690

By the author/editor: Englund, Mikael ...; Caisander, Gunil ...; Noaksson, Karin; Emanuelsson, Kat ...; Lundin, Kersti, ...; Bergh, Christina ...; show more...; Hanson, Charles, ...; Semb, Henrik; Strehl, Raimund; Hyllner, Johan; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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