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Nonlinear microscop...
Nonlinear microscopy of lipid storage and fibrosis in muscle and liver tissues of mice fed high-fat diets
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- Brackmann, Christian, 1973 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Gabrielsson, Britt, 1957 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Svedberg, Fredrik, 1973 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Holmäng, Agneta, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology,University of Gothenburg
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- Sandberg, Ann-Sofie, 1951 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Enejder, Annika, 1969 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- SPIE-Intl Soc Optical Eng, 2010
- 2010
- English.
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In: Journal of Biomedical Optics. - : SPIE-Intl Soc Optical Eng. - 1083-3668 .- 1560-2281. ; 15:6
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Abstract
Subject headings
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- Hallmarks of high-fat Western diet intake, such as excessive lipid accumulation in skeletal muscle and liver as well as liver fibrosis, are investigated in tissues from mice using nonlinear microscopy, second harmonic generation (SHG), and coherent anti-Stokes Raman scattering (CARS), supported by conventional analysis methods. Two aspects are presented; intake of standard chow versus Western diet, and a comparison between two high-fat Western diets of different polyunsaturated lipid content. CARS microscopy images of intramyocellular lipid droplets in muscle tissue show an increased amount for Western diet compared to standard diet samples. Even stronger diet impact is found for liver samples, where combined CARS and SHG microscopy visualize clear differences in lipid content and collagen fiber development, the latter indicating nonalcoholic fatty liver disease (NAFLD) and steatohepatitis induced at a relatively early stage for Western diet. Characteristic for NAFLD, the fibrous tissue-containing lipids accumulate in larger structures. This is also observed in CARS images of liver samples from two Western-type diets of different polyunsaturated lipid contents. In summary, nonlinear microscopy has strong potential (further promoted by technical advances toward clinical use) for detection and characterization of steatohepatitis already in its early stages.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)
Keyword
- cellular biophysics
- coherent antiStokes Raman scattering
- diseases
- harmonic generation
- lipid bilayers
- liver
- muscle
- optical microscopy
- harmonic generation
Publication and Content Type
- ref (subject category)
- art (subject category)
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