Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-duration of symptoms significantly affects detection rate.

• Nucleic acid amplification techniques have improved the diagnostic possibilities in respiratory tract infections, although their clinical applicability is not yet fully defined. We have evaluated a multiplex real-time PCR method for the detection of 13 respiratory viruses and 2 bacteria (Mycoplasma and Chlamydophila) in a clinical setting.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)

Keyword

Adult

Aged

Aged

80 and over

Bacteria

classification

isolation & purification

Bacterial Infections

diagnosis

Community-Acquired Infections

diagnosis

Female

Humans

Male

Middle Aged

Nasopharynx

microbiology

virology

Polymerase Chain Reaction

methods

Prospective Studies

Respiratory Tract Infections

diagnosis

Time Factors

Virus Diseases

diagnosis

Viruses

classification

isolation & purification

Young Adult

Publication and Content Type

ref (subject category)

art (subject category)