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Differentiation of ...
Differentiation of species of the Streptococcus bovis/equinus-complex by MALDI-TOF Mass Spectrometry in comparison to sodA sequence analyses
- Article/chapterEnglish2011
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LIBRIS-ID:oai:gup.ub.gu.se/135046
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https://gup.ub.gu.se/publication/135046URI
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https://doi.org/10.1016/j.syapm.2010.11.010DOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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The Streptococcus bovis/equinus complex is a heterogeneous group within the group D streptococci with important clinical relevance regarding infective endocarditis, sepsis and colon carcinoma. The taxonomic identification of species and sub-species of this complex, by the standard methods remains difficult. In the present study, we compared the cluster analysis of 88 strains of species of the S. bovis/equinus complex by sequence analysis of the manganese-dependent superoxide dismutase gene (sodA) and by Matrix Assisted Laser Desorption/Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS). We observed a high congruence of strain grouping by MALDI-TOF MS in comparison with sodA sequence analyses, demonstrating the accuracy and reliability of MALDI-TOF MS in comparison to DNA sequencebased method. By generating mass spectra for each species and sub-species, we were able to discriminate all members of the S. bovis/equinus complex. Furthermore,wedemonstrated reliable identifications to the species level by MALDI-TOF MS, independently of cultivation conditions.
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Vollmer, T
(author)
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Erhard, M
(author)
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Welker, M
(author)
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Moore, Edward R.B.1954Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xmooed
(author)
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Kleesiek, K
(author)
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Dreier, J
(author)
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Göteborgs universitetInstitutionen för biomedicin, avdelningen för infektionssjukdomar
(creator_code:org_t)
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In:Systematic and Applied Microbiology: Elsevier BV34:1, s. 52-570723-2020
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