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- Munoz, R (author)
Evaluation of Matrix-Assisted Laser Desorption Ionization-Time Of Flight whole cell profiles for assessing the cultivable diversity of aerobic and moderately halophilic prokaryotes thriving in solar saltern sediments
- Article/chapterEnglish2011
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- Elsevier BV,2011
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- LIBRIS-ID:oai:gup.ub.gu.se/135047
- https://gup.ub.gu.se/publication/135047URI
- https://doi.org/10.1016/j.syapm.2010.11.012DOI
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- Language:English
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- The moderately halophilic, cultivable fraction of prokaryotes thriving in hyper-saline sediments of a solar saltern in Mallorca, Spain, has been studied by means of different cultivation media. A set of 374 isolates retrieved with six different culture conditions was screened, using whole-cell MALDI-TOF MS analysis to classify them into 25 phenotypic clusters at 52% similarity. The phylogenetic inference, made from comparative sequence analyses of the 16S rRNA genes of selected strains, indicated that each phenotypic cluster was comprised of a genealogically homogeneous set of strains. DNA–DNA hybridization (DDH) results among selected strains confirmed that each MALDI-TOF cluster encompassed members of the same species. On the other hand, the intra-cluster diversity, measured by several RAPD (Random Amplified Polymorphic DNA) amplifications, indicated that the clusters corresponded to several populations of the same phylogenetic unit coexisting in the same environment. The results encourage the use of MALDI-TOF MS for further exhaustive studies of the cultivable diversity of hyper-saline environments.
Subject headings and genre
- NATURVETENSKAP Kemi Analytisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
- NATURVETENSKAP Biologi Mikrobiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- microbial diversity
- microbial identification
- MALDI-TOF
- mass spectrometry
- microbial cultivation
- RAPD
- DNA-DNA hybridisation
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- López-López, A (author)
- Urdiain, M (author)
- Moore, Edward R.B.1954Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xmooed (author)
- Rosselló-Móra, R (author)
- Göteborgs universitetInstitutionen för biomedicin, avdelningen för infektionssjukdomar (creator_code:org_t)
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- In:Systematic and Applied Microbiology: Elsevier BV34:1, s. 69-750723-2020
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