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High-throughput and high-sensitivity nano-LC/MS and MS/MS for O-glycan profiling.
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- Karlsson, Hasse, 1943 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Holmén Larsson, Jessica, 1971 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Thomsson, Kristina A, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- (creator_code:org_t)
- 2008-02-20
- 2009
- English.
- In: Methods in molecular biology (Clifton, N.J.). - Totowa, NJ : Humana Press. - 1064-3745. ; 534, s. 117-31
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Sensitive and fast methods for the profiling of biologically important molecules are highly demanded. Mucins are densely O-glycosylated glycoproteins found at mucosal surfaces and are of great medical interest. Here we describe sensitive methods for the analysis of O-glycans from mucins using gel electrophoresis, and chromatography by nanoLC on graphite columns and structural analysis by electrospray mass spectrometry on a linear trap mass spectrometer.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- Blotting
- Western
- methods
- Carbohydrate Sequence
- Chromatography
- Liquid
- instrumentation
- methods
- Electrophoresis
- Polyacrylamide Gel
- methods
- Glycomics
- methods
- Humans
- Molecular Sequence Data
- Mucins
- analysis
- chemistry
- isolation & purification
- Nanotechnology
- instrumentation
- methods
- Polysaccharides
- analysis
- chemistry
- isolation & purification
- Sensitivity and Specificity
- Tandem Mass Spectrometry
- instrumentation
- methods
Publication and Content Type
- ref (subject category)
- art (subject category)
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- Methods in molecular biology (Clifton, N.J.) (Search for host publication in LIBRIS)
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