Search: onr:"swepub:oai:gup.ub.gu.se/142191" >
IL-2 induces a WAVE...
IL-2 induces a WAVE2-dependent pathway for actin reorganization that enables WASp-independent human NK cell function.
-
Orange, Jordan S (author)
-
Roy-Ghanta, Sumita (author)
-
Mace, Emily M (author)
-
show more...
-
Maru, Saumya (author)
-
Rak, Gregory D (author)
-
Sanborn, Keri B (author)
-
- Fasth, Anders, 1945 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics
-
Saltzman, Rushani (author)
-
Paisley, Allison (author)
-
Monaco-Shawver, Linda (author)
-
Banerjee, Pinaki P (author)
-
Pandey, Rahul (author)
-
show less...
-
(creator_code:org_t)
- 2011
- 2011
- English.
-
In: The Journal of clinical investigation. - 1558-8238. ; 121:4, s. 1535-48
- Related links:
-
https://gup.ub.gu.se...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Wiskott-Aldrich syndrome (WAS) is a primary immunodeficiency associated with an increased susceptibility to herpesvirus infection and hematologic malignancy as well as a deficiency of NK cell function. It is caused by defective WAS protein (WASp). WASp facilitates filamentous actin (F-actin) branching and is required for F-actin accumulation at the NK cell immunological synapse and NK cell cytotoxicity ex vivo. Importantly, the function of WASp-deficient NK cells can be restored in vitro after exposure to IL-2, but the mechanisms underlying this remain unknown. Using a WASp inhibitor as well as cells from patients with WAS, we have defined a direct effect of IL-2 signaling upon F-actin that is independent of WASp function. We found that IL-2 treatment of a patient with WAS enhanced the cytotoxicity of their NK cells and the F-actin content at the immunological synapses formed by their NK cells. IL-2 stimulation of NK cells in vitro activated the WASp homolog WAVE2, which was required for inducing WASp-independent NK cell function, but not for baseline activity. Thus, WAVE2 and WASp define parallel pathways to F-actin reorganization and function in human NK cells; although WAVE2 was not required for NK cell innate function, it was accessible through adaptive immunity via IL-2. These results demonstrate how overlapping cytoskeletal activities can utilize immunologically distinct pathways to achieve synonymous immune function.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Pediatrik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Pediatrics (hsv//eng)
Keyword
- Wiskott Aldrich syndrome
- primary immunodeficiency
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Orange, Jordan S
-
Roy-Ghanta, Sumi ...
-
Mace, Emily M
-
Maru, Saumya
-
Rak, Gregory D
-
Sanborn, Keri B
-
show more...
-
Fasth, Anders, 1 ...
-
Saltzman, Rushan ...
-
Paisley, Allison
-
Monaco-Shawver, ...
-
Banerjee, Pinaki ...
-
Pandey, Rahul
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Pediatrics
- Articles in the publication
-
The Journal of c ...
- By the university
-
University of Gothenburg