onr:"swepub:oai:gup.ub.gu.se/144015"
Search: onr:"swepub:oai:gup.ub.gu.se/144015" > Identification of N...
- 1 of 1
- Previous record
- Next record
- To hitlist
Identification of Novel α-Synuclein Isoforms in Human Brain Tissue by using an Online NanoLC-ESI-FTICR-MS Method.
-
- Öhrfelt Olsson, Annika, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
-
- Zetterberg, Henrik, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
-
- Persson, Rita, 1951 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
- show more...
- Mulugeta, Ezra (author)
- Francis, Paul T (author)
- Vanmechelen, Eugeen (author)
- Ballard, Clive (author)
- Andersson, K (author)
- show less...
- (creator_code:org_t)
- 2011-06-16
- 2011
- English.
- In: Neurochemical research. - : Springer Science and Business Media LLC. - 1573-6903 .- 0364-3190. ; 36:11, s. 2029-2042
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Parkinson's disease (PD) and Dementia with Lewy bodies (DLB) are neurodegenerative diseases that are characterized by intra-neuronal inclusions of Lewy bodies in distinct brain regions. These inclusions consist mainly of aggregated α-synuclein (α-syn) protein. The present study used immunoprecipitation combined with nanoflow liquid chromatography (LC) coupled to high resolution electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry (ESI-FTICR-MS/MS) to determine known and novel isoforms of α-syn in brain tissue homogenates. N-terminally acetylated full-length α-syn (Ac-α-syn(1-140)) and two N-terminally acetylated C-terminally truncated forms of α-syn (Ac-α-syn(1-139) and Ac-α-syn(1-103)) were found. The different forms of α-syn were further studied by Western blotting in brain tissue homogenates from the temporal cortex Brodmann area 36 (BA36) and the dorsolateral prefrontal cortex BA9 derived from controls, patients with DLB and PD with dementia (PDD). Quantification of α-syn in each brain tissue fraction was performed using a novel enzyme-linked immunosorbent assay (ELISA).
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Psykiatri (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Psychiatry (hsv//eng)
Keyword
- Cerebrospinal fluid
- Immunoprecipitation
- Neurodegenerative diseases
- Mass spectrometry
- Synuclein
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Neurochemical research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Öhrfelt Olsson, ...
- Zetterberg, Henr ...
- Persson, Rita, 1 ...
- Secic, Dzemila
- Brinkmalm, Gunna ...
- Wallin, Anders, ...
- show more...
- Mulugeta, Ezra
- Francis, Paul T
- Vanmechelen, Eug ...
- Aarsland, Dag
- Ballard, Clive
- Blennow, Kaj, 19 ...
- Brinkmalm-Westma ...
- Andersson, K
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Clinical Medicin ...
- and Psychiatry
- Articles in the publication
- Neurochemical re ...
- By the university
- University of Gothenburg
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
