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Comprehensive analy...
Comprehensive analysis of mammalian miRNA* species and their role in myeloid cells.
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Kuchenbauer, Florian (author)
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Mah, Sarah M (author)
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Heuser, Michael (author)
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McPherson, Andrew (author)
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Rüschmann, Jens (author)
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Rouhi, Arefeh (author)
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Berg, Tobias (author)
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Bullinger, Lars (author)
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Argiropoulos, Bob (author)
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Morin, Ryan D (author)
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Lai, David (author)
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Starczynowski, Daniel T (author)
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Karsan, Aly (author)
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Eaves, Connie J (author)
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Watahiki, Akira (author)
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Wang, Yuzhuo (author)
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Aparicio, Samuel A (author)
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Ganser, Arnold (author)
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Krauter, Jürgen (author)
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Döhner, Hartmut (author)
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Döhner, Konstanze (author)
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Marra, Marco A (author)
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Camargo, Fernando D (author)
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- Palmqvist, Lars, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
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Buske, Christian (author)
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Humphries, R Keith (author)
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(creator_code:org_t)
- American Society of Hematology, 2011
- 2011
- English.
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In: Blood. - : American Society of Hematology. - 1528-0020 .- 0006-4971. ; 118:12, s. 3350-8
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
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- Processing of pre-miRNA through Dicer1 generates an miRNA duplex that consists of an miRNA and miRNA* strand. Despite the general view that miRNA*s have no functional role, we further investigated miRNA* species in 10 deep-sequencing libraries from mouse and human tissue. Comparisons of miRNA/miRNA* ratios across the miRNA sequence libraries revealed that 50% of the investigated miRNA duplexes exhibited a highly dominant strand. Conversely, 10% of miRNA duplexes showed a comparable expression of both strands, whereas the remaining 40% exhibited variable ratios across the examined libraries, as exemplified by miR-223/miR-223* in murine and human cell lines. Functional analyses revealed a regulatory role for miR-223* in myeloid progenitor cells, which implies an active role for both arms of the miR-223 duplex. This was further underscored by the demonstration that miR-223 and miR-223* targeted the insulin-like growth factor 1 receptor/phosphatidylinositol 3-kinase axis and that high miR-223* levels were associated with increased overall survival in patients with acute myeloid leukemia. Thus, we found a supporting role for miR-223* in differentiating myeloid cells in normal and leukemic cell states. The fact that the miR-223 duplex acts through both arms extends the complexity of miRNA-directed gene regulation of this myeloid key miRNA.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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Blood
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- By the author/editor
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Kuchenbauer, Flo ...
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Mah, Sarah M
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Heuser, Michael
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McPherson, Andre ...
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Rüschmann, Jens
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Rouhi, Arefeh
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show more...
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Berg, Tobias
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Bullinger, Lars
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Argiropoulos, Bo ...
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Morin, Ryan D
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Lai, David
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Starczynowski, D ...
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Karsan, Aly
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Eaves, Connie J
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Watahiki, Akira
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Wang, Yuzhuo
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Aparicio, Samuel ...
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Ganser, Arnold
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Krauter, Jürgen
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Döhner, Hartmut
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Döhner, Konstanz ...
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Marra, Marco A
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Camargo, Fernand ...
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Palmqvist, Lars, ...
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Buske, Christian
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Humphries, R Kei ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Hematology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
- Articles in the publication
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Blood
- By the university
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University of Gothenburg