Search: onr:"swepub:oai:gup.ub.gu.se/148535" >
The Ashbya gossypii...
The Ashbya gossypiiEF-1α promoter of the ubiquitously used MX cassettes is toxic to Saccharomyces cerevisiae.
-
- Babazadeh, Roja (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi, mikrobiologi,Department of Cell and Molecular Biology, Microbiology,University of Gothenburg
-
Moghadas Jafari, Soode (author)
-
- Zackrisson, Martin (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi, mikrobiologi,Department of Cell and Molecular Biology, Microbiology,University of Gothenburg
-
show more...
-
- Blomberg, Anders, 1956 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi, mikrobiologi,Department of Cell and Molecular Biology, Microbiology,University of Gothenburg
-
- Hohmann, Stefan, 1956 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi, mikrobiologi,Department of Cell and Molecular Biology, Microbiology,University of Gothenburg
-
- Warringer, Jonas, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,University of Gothenburg
-
- Krantz, Marcus, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi, mikrobiologi,Department of Cell and Molecular Biology, Microbiology,University of Gothenburg
-
show less...
-
(creator_code:org_t)
- 2011-10-22
- 2011
- English.
-
In: FEBS letters. - : Wiley. - 1873-3468 .- 0014-5793.
- Related links:
-
https://febs.onlinel...
-
show more...
-
http://dx.doi.org/10...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://research.cha...
-
show less...
Abstract
Subject headings
Close
- Protein overexpression based on introduction of multiple gene copies is well established. To improve purification or quantification, proteins are typically fused to peptide tags. In Saccharomyces cerevisiae, this has been hampered by multicopy toxicity of the TAP and GFP cassettes used in the global strain collections. Here, we show that this effect is due to the EF-1α promoter in the HIS3MX marker cassette rather than the tags per se. This promoter is frequently used in heterologous marker cassettes, including HIS3MX, KanMX, NatMX, PatMX and HphMX. Toxicity could be eliminated by promoter replacement or exclusion of the marker cassette. To our knowledge, this is the first report of toxicity caused by introduction of a heterologous promoter alone.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Histidine toxicity Protein overexpression HIS3 MX toxicity TAP-tag toxicity
- Histidine toxicity Protein overexpression HIS3 MX toxicity TAP-tag toxicity
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database