• Surface mediated immune complement activation can be detected by a variety of antibody utilizing methods such as ELISA, fluorescence- or radiolabelling techniques, QCM, and ellipsometry. In the present work we investigated how the common anticoagulants heparin, dalteparin, fondaparinux and sodium citrate affected the binding of anti-complement factor 3c (anti-C3c) on a model complement activator surface, immobilised IgG, after incubation in human blood serum. The results show, as expected, that different anticoagulants affect the antibody binding differently. Increasing amounts of heparin, dalteparin and sodium citrate in normal serum resulted in a decreasing anti-C3c binding. The antibody deposition was not sensitive for the fondaparinux concentration. Surprisingly high concentrations of anti-coagulantia were needed to completely eradicate the antibody binding. Experiments in EGTA-serum showed that anticoagulants interfered directly with both the classical and alternative pathways. Control C3a-des arg ELISA measurements show that the lowered antibody surface binding was not a result of complement depletion in serum. Kallikrein generation by hydrophilic glass surfaces was not affected by high anticoagulant concentrations.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Kirurgi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Surgery (hsv//eng)

Keyword

Anticoagulants

blood

pharmacology

Blood Proteins

metabolism

Colorimetry

Complement Activation

drug effects

Complement C3

metabolism

Complement C3a

metabolism

Complement System Proteins

analysis

Dalteparin

pharmacology

Egtazic Acid

diagnostic use

Enzyme-Linked Immunosorbent Assay

Heparin

pharmacology

Humans

Immunoassay

Immunoglobulin G

chemistry

Indicators and Reagents

Kallikreins

Molecular Weight

Polysaccharides

pharmacology

Surface Plasmon Resonance

Surface Properties

NATURAL SCIENCES

Publication and Content Type

ref (subject category)

art (subject category)