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Straightforward FRA...
Straightforward FRAP for quantitative diffusion measurements with a laser scanning microscope
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- Deschout, Hendrik (author)
- Universiteit Gent,Ghent university
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- Hagman, Joel H, 1983 (author)
- RISE,SIK – Institutet för livsmedel och bioteknik
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- Fransson, Sophia, 1979 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Jonasson, Jenny, 1976 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper, matematisk statistik,Department of Mathematical Sciences, Mathematical Statistics,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg
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- Rudemo, Mats, 1937 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper, matematisk statistik,Department of Mathematical Sciences, Mathematical Statistics,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology
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- Lorén, Niklas, 1970 (author)
- RISE,SIK – Institutet för livsmedel och bioteknik
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- Braeckmans, Kevin (author)
- Universiteit Gent,Ghent university
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(creator_code:org_t)
- 2010
- 2010
- English.
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In: Optics Express. - 1094-4087. ; 18:22, s. 22886-22905
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Abstract
Subject headings
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- Confocal or multi-photon laser scanning microscopes are convenient tools to perform FRAP diffusion measurements. Despite its popularity, accurate FRAP remains often challenging since current methods are either limited to relatively large bleach regions or can be complicated for non-specialists. In order to bring reliable quantitative FRAP measurements to the broad community of laser scanning microscopy users, here we have revised FRAP theory and present a new pixel based FRAP method relying on the photo bleaching of rectangular regions of any size and aspect ratio. The method allows for fast and straightforward quantitative diffusion measurements due to a closed–form expression for the recovery process utilizing all available spatial and temporal data. After a detailed validation, its versatility is demonstrated by diffusion studies in heterogeneous biopolymer mixtures.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk laboratorie- och mätteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Laboratory and Measurements Technologies (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
Keyword
- Medical optics and biotechnology
- Confocal microscopy
- Microscopy
- Fluorescence microscopy
- Fluorescence microscopy
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Deschout, Hendri ...
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Hagman, Joel H, ...
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Fransson, Sophia ...
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Jonasson, Jenny, ...
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Rudemo, Mats, 19 ...
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Lorén, Niklas, 1 ...
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show more...
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Braeckmans, Kevi ...
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- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Medical Engineer ...
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and Medical Laborato ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agriculture Fore ...
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and Food Science
- Articles in the publication
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Optics Express
- By the university
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University of Gothenburg
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Chalmers University of Technology
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RISE