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An in situ fracture...
An in situ fracture device to image lipids in single cells using ToF-SIMS
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- Lanekoff, Ingela, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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- Kurczy, Michael, 1980 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Adams, Kelly L. (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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Malm, J (author)
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- Karlsson, Roger, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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Sjovall, P (author)
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- Ewing, Andrew G, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
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(creator_code:org_t)
- 2010-06-22
- 2011
- English.
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In: SURFACE AND INTERFACE ANALYSIS. - : Wiley. - 0142-2421 .- 1096-9918. ; 43:1-2, s. 257-260
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Abstract
Subject headings
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- Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) imaging yields molecule-specific images showing the spatial distribution of specific lipids with submicron resolution, making it a potentially powerful method for studying a variety of biological questions in single cells. In order to make possible the analysis of hydrated cells in vacuum, we have designed a device for in situ freeze-fracture of cell samples. PC12 cells are frozen between two silicon shards in a sandwich geometry, and the fracture is conducted under vacuum inside the analysis chamber of the instrument. After freeze-fracture, ToF-SIMS analysis was carried out using a Bi cluster ion source, providing high-resolution images of single cells. We also show that when combining freeze-fracture and cluster SIMS imaging, low abundant biologically important lipids, specifically PE, PC and non-SM PC, can be imaged in single cells. Finally, K+ ion localization was used as a diagnostic to identify fracture planes through the cell for these fractured samples.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- tof-sims imaging
- pc12 cells
- freeze-fracture
- phospholipids
- pc12 cells
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Lanekoff, Ingela ...
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Kurczy, Michael, ...
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Adams, Kelly L.
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Malm, J
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Karlsson, Roger, ...
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Sjovall, P
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show more...
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Ewing, Andrew G, ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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SURFACE AND INTE ...
- By the university
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University of Gothenburg
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Chalmers University of Technology
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Uppsala University