Chemical images of marine bio-active compounds by surface enhanced Raman spectroscopy and transposed orthogonal partial least squares (T-OPLS)

Surface enhanced Raman spectroscopy combined with transposed Orthogonal Partial Least Squares (T-OPLS) was shown to produce chemical images of the natural antibacterial surface-active compound 1,1,3,3-tetrabromo-2-heptanone (TBH) on Bonnemaisonia hamifera. The use of gold colloids function-alised with the internal standard 4-mercapto-benzonitrile (MBN) made it possible to create images of the relative concentration of TBH over the surfaces. A gradient of TBH could be mapped over and in the close vicinity of the B. hamifera algal vesicles at the attomol/pixel level. T-OPLS produced a measure of the spectral correlation for each pixel of the hyperspectral images whilst not including spectral variation that was linearly independent of the target spectrum. In this paper we show the possibility to retrieve specific spectral information with a low magnitude in a complex matrix.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Biologi Ekologi hsv//swe
NATURAL SCIENCES Biological Sciences Ecology hsv//eng
Surface enhanced Raman spectroscopy
Bonnemaisonia hamifera 1
1
3
3-Tetrabromo-2-heptanone
red alga-bonnemaisonia
bacterial-colonization
scattering
pls
chemometrics
fluorescence
feasibility
information
hamifera
information

Josefson, M.AstraZeneca R&D Mölndal (author)
Nylund, Göran M.,1974Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap, Tjärnö marinbiologiska laboratorium,Linnécentrum för marin evolutionsbiologi (CEMEB),Centrum för marin kemisk ekologi,Department of Biological and Environmental Sciences, Tjärnö Marine Biological Laboratory,Linnaeus Centre for Marine Evolutionary Biology (CEMEB),Centre for Marine Chemical Ecology,University of Gothenburg(Swepub:gu)xnylgo (author)
Pavia, Henrik,1964Gothenburg University,Göteborgs universitet,Institutionen för biologi och miljövetenskap, Tjärnö marinbiologiska laboratorium,Centrum för marin kemisk ekologi,Linnécentrum för marin evolutionsbiologi (CEMEB),Department of Biological and Environmental Sciences, Tjärnö Marine Biological Laboratory,Centre for Marine Chemical Ecology,Linnaeus Centre for Marine Evolutionary Biology (CEMEB),University of Gothenburg(Swepub:gu)xpavhe (author)
Abrahamsson, Katarina,1957Göteborgs universitet,University of Gothenburg(Swepub:cth)xabrak (author)
Göteborgs universitetAstraZeneca R&D Mölndal (creator_code:org_t)

By the author/editor: Abbas, Aamer, 19 ...; Josefson, M.; Nylund, Göran M. ...; Pavia, Henrik, 1 ...; Abrahamsson, Kat ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

By the university: University of Gothenburg; Chalmers University of Technology

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