onr:"swepub:oai:gup.ub.gu.se/162799"
Search: onr:"swepub:oai:gup.ub.gu.se/162799" > Brain nitric oxide:...
- 1 of 1
- Previous record
- Next record
- To hitlist
Brain nitric oxide: Regional characterisation of a real-time microelectrochemical sensor
- Finnerty, N. J. (author)
- O'Riordan, S. L. (author)
-
- Pålsson, Erik, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
- show more...
- Lowry, J. P. (author)
- show less...
- (creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
- In: Journal of Neuroscience Methods. - : Elsevier BV. - 0165-0270. ; 209:1, s. 13-21
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- A reliable method of directly measuring endogenously generated nitric oxide (NO) in real-time and in various brain regions is presented. An extensive characterisation of a previously described amperometric sensor has been carried out in the prefrontal cortex and nucleus accumbens of freely moving rats. Systemic administration of saline caused a transient increase in signal from baseline levels in both the prefrontal cortex (13 +/- 3 pA, n = 17) and nucleus accumbens (12 +/- 3 pA, n = 8). NO levels in the prefrontal cortex were significantly increased by 43 +/- 9 pA (n = 9) following administration of L-arginine. A similar trend was observed in the nucleus accumbens, where an increase of 44 +/- 9 pA (n = 8) was observed when compared against baseline levels. Systemic injections of the non-selective NOS inhibitor L-NAME produced a significant decrease in current recorded in the prefrontal cortex (24 +/- 6 pA, n = 5) and nucleus accumbens (17 +/- 3 pA, n = 6). Finally it was necessary to validate the sensors functionality in vivo by investigating the effect of the interferent ascorbate on the oxidation current. The current showed no variation in both regions over the selected time interval of 60 min, indicating no deterioration of the polymer membrane. A detailed comparison identified significantly greater affects of administrations on NO sensors implanted in the striatum than those inserted in the prefrontal cortex and the nucleus accumbens. (c) 2012 Elsevier B.V. All rights reserved.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Nitric oxide
- Real-time
- Prefrontal cortex
- Nucleus accumbens
- In vivo
- cerebral-blood-flow
- phencyclidine-induced disruption
- methyl-d-aspartate
- cyclic-gmp levels
- in-vivo
- l-arginine
- prepulse
- inhibition
- synthase inhibitors
- rat-brain
- nucleus-accumbens
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Journal of Neuroscience Methods (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Finnerty, N. J.
- O'Riordan, S. L.
- Pålsson, Erik, 1 ...
- Lowry, J. P.
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Neurosciences
- Articles in the publication
- Journal of Neuro ...
- By the university
- University of Gothenburg
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
