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CO2 permeability of...
CO2 permeability of cell membranes is regulated by membrane cholesterol and protein gas channels
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Itel, F. (author)
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Al-Samir, S. (author)
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- Öberg, Fredrik, 1982 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Chami, M. (author)
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Kumar, M. (author)
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Supuran, C. T. (author)
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Deen, P. M. T. (author)
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Meier, W. (author)
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- Hedfalk, Kristina, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Gros, G. (author)
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Endeward, V. (author)
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(creator_code:org_t)
- 2012-09-10
- 2012
- English.
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In: Faseb Journal. - : Wiley. - 0892-6638 .- 1530-6860. ; 26:12, s. 5182-5191
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https://doi.org/10.1...
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Abstract
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- Recent observations that some membrane proteins act as gas channels seem surprising in view of the classical concept that membranes generally are highly permeable to gases. Here, we study the gas permeability of membranes for the case of CO2, using a previously established mass spectrometric technique. We first show that biological membranes lacking protein gas channels but containing normal amounts of cholesterol (30-50 mol% of total lipid), e.g., MDCK and tsA201 cells, in fact possess an unexpectedly low CO2 permeability (PCO2) of similar to 0.01 cm/s, which is 2 orders of magnitude lower than the PCO2 of pure planar phospholipid bilayers (similar to 1 cm/s). Phospholipid vesicles enriched with similar amounts of cholesterol also exhibit PCO2 approximate to 0.01 cm/s, identifying cholesterol as the major determinant of membrane PCO2. This is confirmed by the demonstration that MDCK cells depleted of or enriched with membrane cholesterol show dramatic increases or decreases in PCO2, respectively. We demonstrate, furthermore, that reconstitution of human AQP-1 into cholesterol-containing vesicles, as well as expression of human AQP-1 in MDCK cells, leads to drastic increases in PCO2, indicating that gas channels are of high functional significance for gas transfer across membranes of low intrinsic gas permeability.-Itel, F., Al-Samir, S., Oberg, F., Chami, M., Kumar, M., Supuran, C. T., Deen, P. M. T., Meier, W., Hedfalk, K., Gros, G., Endeward, V. CO2 permeability of cell membranes is regulated by membrane cholesterol and protein gas channels. FASEB J. 26, 5182-5191 (2012). www.fasebj.org
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- aquaporin 1
- aquaporin Z
- phospholipid vesicles
- MDCK cells
- lipid bilayer-membranes
- carbon-dioxide permeability
- human
- erythrocyte-membrane
- red-blood-cells
- water channel
- epithelial
- membrane
- normal-decane
- aquaporin-1
- transport
- liposomes
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Itel, F.
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Al-Samir, S.
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Öberg, Fredrik, ...
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Chami, M.
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Kumar, M.
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Supuran, C. T.
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show more...
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Deen, P. M. T.
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Meier, W.
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Hedfalk, Kristin ...
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Gros, G.
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Endeward, V.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Faseb Journal
- By the university
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University of Gothenburg