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Induction of resist...
Induction of resistance to Aplidin in a human ovarian cancer cell line related to MDR expression.
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- Tognon, Gianluca, 1976 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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Bernasconi, Sergio (author)
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Celli, Nicola (author)
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Faircloth, Glynn T (author)
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Cuevas, Carmen (author)
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Jimeno, José (author)
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Erba, Eugenio (author)
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D'Incalci, Maurizio (author)
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(creator_code:org_t)
- 2005
- 2005
- English.
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In: Cancer biology & therapy. - 1538-4047. ; 4:12, s. 1325-30
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- Aplidin-resistant IGROV-1/APL cells were derived from the human ovarian cancer IGROV-1 cell line by exposing the cells to increasing concentration of Aplidin for eight months, starting from a concentration of 10 nM to a final concentration of 4 microM. IGROV-1/APL cell line possesses five fold relative resistance to Aplidin. IGROV-1/APL resistant cell line shows the typical MDR phenotype: (1) increased expression of membrane-associated P-glycoprotein, (2) cross-resistance to drugs like etoposide, doxorubicin, vinblastine, vincristine, taxol, colchicin and the novel anticancer drug Yondelis (ET-743). The Pgp inhibitor cyclosporin-A restored the sensitivity of IGROV-1/APL cells to Aplidin by increasing the drug intracellular concentration. The resistance to Aplidin was not due to the other proteins, such as LPR-1 and MRP-1, being expressed at the same level in resistant and parental cell line. The finding that cells over-expressing Pgp are resistant to Aplidin was confirmed in CEM/VLB 100 cells, that was found to be 5-fold resistant to Aplidin compared to the CEM parental cell line.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- Antibiotics
- Antineoplastic
- pharmacology
- Antineoplastic Agents
- pharmacology
- Antineoplastic Agents
- Alkylating
- pharmacology
- Antineoplastic Agents
- Phytogenic
- pharmacology
- Cell Line
- Tumor
- Cyclosporine
- pharmacology
- Depsipeptides
- pharmacology
- Dose-Response Relationship
- Drug
- Drug Resistance
- Multiple
- Drug Resistance
- Neoplasm
- Enzyme Inhibitors
- pharmacology
- Female
- Genes
- MDR
- Humans
- Isoquinolines
- pharmacology
- Ovarian Neoplasms
- metabolism
- pathology
- P-Glycoprotein
- metabolism
- Peptides
- Cyclic
- pharmacology
Publication and Content Type
- ref (subject category)
- art (subject category)
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