Search: onr:"swepub:oai:gup.ub.gu.se/172440" >
Natively inhibited ...
Natively inhibited Trypanosoma brucei cathepsin B structure determined by using an X-ray laser.
-
Redecke, Lars (author)
-
Nass, Karol (author)
-
DePonte, Daniel P (author)
-
show more...
-
White, Thomas A (author)
-
Rehders, Dirk (author)
-
Barty, Anton (author)
-
Stellato, Francesco (author)
-
Liang, Mengning (author)
-
Barends, Thomas R M (author)
-
Boutet, Sébastien (author)
-
Williams, Garth J (author)
-
- Seibert, M Marvin (author)
- Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.
-
Seibert, M Marvin (author)
-
Aquila, Andrew (author)
-
- Arnlund, David (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Bajt, Sasa (author)
-
Barth, Torsten (author)
-
Bogan, Michael J (author)
-
- Caleman, Carl (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Chao, Tzu-Chiao (author)
-
Doak, R Bruce (author)
-
Fleckenstein, Holger (author)
-
Frank, Matthias (author)
-
Fromme, Raimund (author)
-
Galli, Lorenzo (author)
-
Grotjohann, Ingo (author)
-
Hunter, Mark S (author)
-
- Johansson, Linda C, 1983 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Kassemeyer, Stephan (author)
-
- Katona, Gergely, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Kirian, Richard A (author)
-
Koopmann, Rudolf (author)
-
Kupitz, Chris (author)
-
Lomb, Lukas (author)
-
Martin, Andrew V (author)
-
Mogk, Stefan (author)
-
- Neutze, Richard, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Shoeman, Robert L (author)
-
Steinbrener, Jan (author)
-
- Timneanu, Nicusor (author)
- Uppsala universitet,Molekylär biofysik
-
Wang, Dingjie (author)
-
Weierstall, Uwe (author)
-
Zatsepin, Nadia A (author)
-
Spence, John C H (author)
-
Fromme, Petra (author)
-
Schlichting, Ilme (author)
-
Duszenko, Michael (author)
-
Betzel, Christian (author)
-
Chapman, Henry N (author)
-
show less...
-
Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA Institutionen för kemi och molekylärbiologi (creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2013
- 2013
- English.
-
In: Science (New York, N.Y.). - : American Association for the Advancement of Science (AAAS). - 1095-9203 .- 0036-8075. ; 339:6116, s. 227-30
- Related links:
-
https://europepmc.or...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- The Trypanosoma brucei cysteine protease cathepsin B (TbCatB), which is involved in host protein degradation, is a promising target to develop new treatments against sleeping sickness, a fatal disease caused by this protozoan parasite. The structure of the mature, active form of TbCatB has so far not provided sufficient information for the design of a safe and specific drug against T. brucei. By combining two recent innovations, in vivo crystallization and serial femtosecond crystallography, we obtained the room-temperature 2.1 angstrom resolution structure of the fully glycosylated precursor complex of TbCatB. The structure reveals the mechanism of native TbCatB inhibition and demonstrates that new biomolecular information can be obtained by the "diffraction-before-destruction" approach of x-ray free-electron lasers from hundreds of thousands of individual microcrystals.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Keyword
- Amino Acid Sequence
- Animals
- Catalytic Domain
- Cathepsin B
- antagonists & inhibitors
- chemistry
- Crystallization
- Crystallography
- X-Ray
- Enzyme Precursors
- chemistry
- Glycosylation
- Models
- Molecular
- Molecular Sequence Data
- Protein Conformation
- Protozoan Proteins
- antagonists & inhibitors
- chemistry
- Sf9 Cells
- Spodoptera
- Trypanosoma brucei brucei
- enzymology
- X-Rays
- NATURAL SCIENCES
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Redecke, Lars
-
Nass, Karol
-
DePonte, Daniel ...
-
White, Thomas A
-
Rehders, Dirk
-
Barty, Anton
-
show more...
-
Stellato, France ...
-
Liang, Mengning
-
Barends, Thomas ...
-
Boutet, Sébastie ...
-
Williams, Garth ...
-
Seibert, M Marvi ...
-
Aquila, Andrew
-
Arnlund, David
-
Bajt, Sasa
-
Barth, Torsten
-
Bogan, Michael J
-
Caleman, Carl
-
Chao, Tzu-Chiao
-
Doak, R Bruce
-
Fleckenstein, Ho ...
-
Frank, Matthias
-
Fromme, Raimund
-
Galli, Lorenzo
-
Grotjohann, Ingo
-
Hunter, Mark S
-
Johansson, Linda ...
-
Kassemeyer, Step ...
-
Katona, Gergely, ...
-
Kirian, Richard ...
-
Koopmann, Rudolf
-
Kupitz, Chris
-
Lomb, Lukas
-
Martin, Andrew V
-
Mogk, Stefan
-
Neutze, Richard, ...
-
Shoeman, Robert ...
-
Steinbrener, Jan
-
Timneanu, Nicuso ...
-
Wang, Dingjie
-
Weierstall, Uwe
-
Zatsepin, Nadia ...
-
Spence, John C H
-
Fromme, Petra
-
Schlichting, Ilm ...
-
Duszenko, Michae ...
-
Betzel, Christia ...
-
Chapman, Henry N
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biochemistry and ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Structural Biolo ...
- Articles in the publication
-
Science (New Yor ...
- By the university
-
University of Gothenburg
-
Uppsala University