Isolated hyperthermic liver perfusion with cytostatic-containing perfusate activates the complement cascade.

• Eight patients with advanced liver malignancy undergoing isolated hyperthermic liver perfusion with melphalan and cisplatin were studied with regard to complement activation and formation of anaphylatoxins (C3a and C5a) and terminal C5b-9 complement complexes (TCCs). Blood samples for complement variables (C1-INH, C3, C4, C5, C3a, C5a and TCCs) were taken before surgery, 1 min before the start of perfusion, 1, 2 and 3 h after the start of perfusion, and 24 h after operation. Samples were drawn from the perfusate 1 h after the start of perfusion. Activation of complement was observed during perfusion. Raised plasma concentrations of C3a and TCCs were recorded and high levels of C3a and TCCs were found in the perfusate. In vitro tests indicated that melphalan and cisplatin may activate complement. This activation occurred at 37 and 42 degrees C but was more pronounced at 42 degrees C.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Keyword

Adult

Aged

Aged

80 and over

Chemotherapy

Cancer

Regional Perfusion

Cisplatin

administration & dosage

Complement Activation

immunology

Complement C3a

analysis

Complement C5a

analysis

Female

Humans

Hyperthermia

Induced

Liver

immunology

Liver Neoplasms

immunology

therapy

Male

Melphalan

analysis

Middle Aged

Publication and Content Type

ref (subject category)

art (subject category)