Evaluation of macrophage-specific promoters using lentiviral delivery in mice.

Levin, Malin, 1973 (author): Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberglaboratoriet,Institute of Medicine, Department of Molecular and Clinical Medicine,Wallenberg Laboratory,University of Gothenburg

Lidberg, Ulf, 1962 (author): Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberg Laboratory,Institute of Medicine, Department of Molecular and Clinical Medicine,University of Gothenburg

Jirholt, Pernilla, 1972 (author): Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research,University of Gothenburg

Adiels, Martin, 1976 (author): Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institutionen för matematiska vetenskaper,Institute of Medicine, Department of Molecular and Clinical Medicine,Department of Mathematical Sciences,Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg

Wramstedt, Anna, 1983 (author): Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory,University of Gothenburg

Gustafsson, K (author)

Greaves, D R (author)

Li, S (author)

Fazio, S (author)

Linton, M F (author)

Olofsson, Sven-Olof, 1947 (author): Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberglaboratoriet,Institute of Medicine, Department of Molecular and Clinical Medicine,Wallenberg Laboratory,University of Gothenburg

Borén, Jan, 1963 (author): Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberg Laboratory,Institute of Medicine, Department of Molecular and Clinical Medicine,University of Gothenburg

Gjertsson, Inger, 1962 (author): Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research,University of Gothenburg

(creator_code:org_t)

2011-12-01
2012
English.
In: Gene therapy. - : Springer Science and Business Media LLC. - 1476-5462 .- 0969-7128. ; 19:11, s. 1041-7

Related links:: https://www.nature.c...; show more...; http://dx.doi.org/10...; https://gup.ub.gu.se...; https://doi.org/10.1...; https://research.cha...; show less...

Abstract Subject headings

In gene therapy, tissue-specific promoters are useful tools to direct transgene expression and improve efficiency and safety. Macrophage-specific promoters (MSPs) have previously been published using different delivery systems. In this study, we evaluated five different MSPs fused with green fluorescent protein (GFP) to delineate the one with highest specificity using lentiviral delivery. We compared three variants of the CD68 promoter (full length, the 343-bp proximal part and the 150-bp proximal part) and two variants (in forward and reverse orientation) of a previously characterized synthetic promoter derived from elements of transcription factor genes. We transduced a number of cell lines and primary cells in vitro. In addition, hematopoietic stem cells were transduced with MSPs and transferred into lethally irradiated recipient mice. Fluorescence activated cell sorting analysis was performed to determine the GFP expression in different cell populations both in vitro and in vivo. We showed that MSPs can efficiently be used for lentiviral gene delivery and that the 150-bp proximal part of the CD68 promoter provides primarily macrophage-specific expression of GFP. We propose that this is the best currently available MSP to use for directing transgene expression to macrophage populations in vivo using lentiviral vectors.

By the author/editor: Levin, Malin, 19 ...; Lidberg, Ulf, 19 ...; Jirholt, Pernill ...; Adiels, Martin, ...; Wramstedt, Anna, ...; Gustafsson, K; show more...; Greaves, D R; Li, S; Fazio, S; Linton, M F; Olofsson, Sven-O ...; Borén, Jan, 1963; Gjertsson, Inger ...; show less...

About the subject

By the university: University of Gothenburg; Chalmers University of Technology

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