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High Resolution Pro...
High Resolution Protein Crystals Using an Efficient Convection-Free Geometry
- Article/chapterEnglish2013
Publisher, publication year, extent ...
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2012-12-24
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American Chemical Society (ACS),2013
Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/175289
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https://gup.ub.gu.se/publication/175289URI
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https://doi.org/10.1021/cg301497tDOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Macromolecular crystallography is the most direct and accurate approach to determine the three-dimensional structure of biological macromolecules. The growth of high quality single crystals, yielding diffraction to the highest X-ray resolution, remains a bottleneck in this methodology. Here we show that through a modification of the batch crystallization method, an entirely convection-free crystallization environment is achieved, which enhances the purity and crystallinity of protein crystals. This is accomplished by using an upside-down geometry, where crystals grow at the “ceiling” of a growth-cell completely filled with the crystallization solution. The “ceiling crystals” experience the same diffusion-limited conditions as in space microgravity experiments. The new method was tested on bovine insulin and two hen egg-white lysozyme polymorphs. In all cases, ceiling crystals diffracted X-rays to resolution limits beyond that for other methods using similar crystallization conditions without further optimization. In addition, we demonstrate that the ceiling crystallization method leads to crystals with much lower impurity incorporation.
Subject headings and genre
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Rebuffet, EtienneGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
(author)
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Törnroth-Horsefield, Susanna,1973Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xtorsu
(author)
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de Grip, Willem J.
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van Enckevort, Willem J. P.
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Vlieg, Elias
(author)
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Göteborgs universitetInstitutionen för kemi och molekylärbiologi
(creator_code:org_t)
Related titles
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In:Crystal Growth & Design: American Chemical Society (ACS)13:2, s. 775-7811528-74831528-7505
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