onr:"swepub:oai:gup.ub.gu.se/175367"
Search: onr:"swepub:oai:gup.ub.gu.se/175367" > The real catecholam...
- 1 of 1
- Previous record
- Next record
- To hitlist
The real catecholamine content of secretory vesicles in the CNS revealed by electrochemical cytometry
-
- Omiatek, D. M. (author)
- Pennsylvania State University
-
- Bressler, A. J. (author)
- Pennsylvania State University
-
- Cans, Ann-Sofie, 1971 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
- show more...
- show less...
- (creator_code:org_t)
- 2013-03-14
- 2013
- English.
- In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 3
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Resolution of synaptic vesicle neurotransmitter content has mostly been limited to the study of stimulated release in cultured cell systems, and it has been controversial as to whether synaptic vesicle transmitter levels are saturated in vivo. We use electrochemical cytometry to count dopamine molecules in individual synaptic vesicles in populations directly sampled from brain tissue. Vesicles from the striatum yield an average of 33,000 dopamine molecules per vesicle, an amount considerably greater than typically measured during quantal release at cultured neurons. Vesicular content was markedly increased by L-DOPA or decreased by reserpine in a time-dependent manner in response to in vivo administration of drugs known to alter dopamine release. We investigated the effects of the psychostimulant amphetamine on vesicle content, finding that vesicular transmitter is rapidly depleted by 50% following in vivo administration, supporting the "weak base hypothesis'' that amphetamine reduces synaptic vesicle transmitter and quantal size.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- substantia-nigra neurons
- ventral tegmental area
- quantal size
- dopamine
- release
- capillary-electrophoresis
- pc12 cells
- rat-brain
- exocytosis
- amphetamine
- midbrain
- rat-brain
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Scientific Reports (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Omiatek, D. M.
- Bressler, A. J.
- Cans, Ann-Sofie, ...
- Andrews, A. M.
- Heien, M. L.
- Ewing, Andrew G, ...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Chemical Science ...
- Articles in the publication
- Scientific Repor ...
- By the university
- University of Gothenburg
- Chalmers University of Technology
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
