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Human pheochromocyt...
Human pheochromocytoma cells studied in culture contain large amounts of DSIP-like material.
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- Nilsson, Ola, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin , Avdelningen för patologi,Institute of Laboratory Medicine, Dept of Pathology
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- Wängberg, Bo, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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Wigander, A (author)
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Lundmark, K (author)
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Dahlström, A (author)
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- Ahlman, Håkan, 1947 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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Bjartell, A (author)
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Ekman, R (author)
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(creator_code:org_t)
- 1991
- 1991
- English.
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In: Peptides. - 0196-9781. ; 12:5, s. 1077-83
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
Close
- Delta sleep-inducing peptide (DSIP)-like immunoreactive (LI) material has been detected in nine different human pheochromocytoma tumors by immunocytochemistry. In primary tumors subjected to indirect immunofluorescence a variable number of tumor cells (25-75%) showed positive cytoplasmic labeling after incubation with DSIP antiserum. Tumor cells grown in culture were strongly labeled by the DSIP antiserum with DSIP-LI concentrated to cell bodies. Electron microscopic immunocytochemistry (immunogold labeling) of pheochromocytoma cells demonstrated DSIP-LI over the dense core of secretory granules. The presence of DSIP-LI in several HPLC fractions from conditioned culture media indicates secretion of DSIP-LI from cultured pheochromocytoma cells. The observations suggest that DSIP-LI is synthesized and stored in secretory granules before release. The different HPLC profiles from each of the tumors may reflect differences in processing or turnover of DSIP-LI in pheochromocytoma cells.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Keyword
- Adrenal Gland Neoplasms
- pathology
- ultrastructure
- Chromatography
- High Pressure Liquid
- Culture Techniques
- methods
- Delta Sleep-Inducing Peptide
- analysis
- Fluorescent Antibody Technique
- Humans
- Microscopy
- Immunoelectron
- Pheochromocytoma
- pathology
- ultrastructure
- Tumor Cells
- Cultured
Publication and Content Type
- ref (subject category)
- art (subject category)
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Peptides
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- By the author/editor
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Nilsson, Ola, 19 ...
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Wängberg, Bo, 19 ...
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Wigander, A
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Lundmark, K
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Dahlström, A
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Ahlman, Håkan, 1 ...
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show more...
-
Bjartell, A
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Ekman, R
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Surgery
- Articles in the publication
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Peptides
- By the university
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University of Gothenburg