Search: onr:"swepub:oai:gup.ub.gu.se/181314" >
NHE8 plays an impor...
NHE8 plays an important role in mucosal protection via its effect on bacterial adhesion.
-
Liu, Chang (author)
-
Xu, Hua (author)
-
Zhang, Bo (author)
-
show more...
-
- Johansson, Malin E V, 1971 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
-
Li, Jing (author)
-
- Hansson, Gunnar C., 1951 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
-
Ghishan, Fayez K (author)
-
show less...
-
(creator_code:org_t)
- American Physiological Society, 2013
- 2013
- English.
-
In: American journal of physiology. Cell physiology. - : American Physiological Society. - 1522-1563 .- 0363-6143. ; 305:1
- Related links:
-
https://europepmc.or...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- The Na(+)/H(+) exchanger NHE8 is expressed on the apical membrane of intestinal epithelial cells and is particularly abundant in the colon. Our previous study showed that Muc2 expression was significantly reduced in NHE8-knockout (NHE8(-/-)) mice, suggesting that NHE8 plays a role in mucosal protection in the colon. The current study confirms and extends our studies on the role of NHE8 in mucosal protection. The number of bacteria attached on the distal colon was significantly increased in NHE8(-/-) mice compared with their wild-type littermates. As expected, IL-4 expression was markedly increased in NHE8(-/-) mice compared with wild-type mice. Immunohistochemistry showed disorganization in the mucin layer of NHE8(-/-) mice, suggesting a possible direct bacteria-epithelia interaction. Furthermore, NHE8(-/-) mice were susceptible to dextran sodium sulfate-induced mucosal injury. In wild-type mice, dextran sodium sulfate treatment inhibited colonic NHE8 expression. In Caco-2 cells, the absence of NHE8 expression resulted in higher adhesion rates of Salmonella typhimurium but not Lactobacillus plantarum. Similarly, in vivo, S. typhimurium adhesion rate was increased in NHE8(-/-) mice compared with wild-type mice. Our study suggests that NHE8 plays important roles in protecting intestinal epithelia from infectious bacterial adherence.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
Keyword
- NHE8
- mucosal layer
- salmonella
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database