Search: onr:"swepub:oai:gup.ub.gu.se/184271" >
Isolectins from Sol...
Isolectins from Solanum tuberosum with different detailed carbohydrate binding specificities: unexpected recognition of lactosylceramide by N-acetyllactosamine-binding lectins.
-
Ciopraga, J (author)
-
- Ångström, Jonas, 1950 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
-
- Bergström, Jörgen, 1953 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
-
show more...
-
- Larsson, Thomas, 1947 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
-
- Karlsson, Niclas G., 1966 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
-
Motas, C (author)
-
Gozia, O (author)
-
- Teneberg, Susann, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk och fysiologisk kemi,Institute of Medical Biochemistry
-
show less...
-
(creator_code:org_t)
- 2000
- 2000
- English.
-
In: Journal of biochemistry. - 0021-924X. ; 128:5, s. 855-67
- Related links:
-
https://gup.ub.gu.se...
Abstract
Subject headings
Close
- Glycosphingolipid recognition by two isolectins from Solanum tuberosum was compared by the chromatogram binding assay. One lectin (PL-I) was isolated from potato tubers by affinity chromatography, and identified by MALDI-TOF mass spectrometry as a homodimer with a subunit molecular mass of 63,000. The other (PL-II) was a commercial lectin, characterized as two homodimeric isolectins with subunit molecular masses of 52,000 and 55,000, respectively. Both lectins recognized N-acetyllactosamine-containing glycosphingolipids, but the fine details of their carbohydrate binding specificities differed. PL-II preferentially bound to glycosphingolipids with N-acetyllactosamine branches, as Galbeta4GlcNAcbeta6(Galbeta4GlcNAcbeta3)Galbeta4Glcbeta1C er. PL-I also recognized this glycosphingolipid, but bound equally well to the linear glycosphingolipid Galbeta4GlcNAcbeta3Galbeta4GlcNAcbeta3Galbeta4Glcbeta1Cer. Neolactotetraosylceramide and the B5 pentaglycosylceramide were also bound by PL-I, while other glycosphingolipids with only one N-acetyllactosamine unit were non-binding. Surprisingly, both lectins also bound to lactosylceramide, with an absolute requirement for sphingosine and non-hydroxy fatty acids. The inhibition of binding to both lactosylceramide and N-acetyllactosamine-containing glycosphingolipids by N-acetylchitotetraose suggests that lactosylceramide is also accomodated within the N-acetylchitotetraose/N-acetyllactosamine-binding sites of the lectins. Through docking of glycosphingolipids onto a three-dimensional model of the PL-I hevein binding domain, a Galbeta4GlcNAcbeta3Galbeta4 binding epitope was defined. Furthermore, direct involvement of the ceramide in the binding of lactosylceramide was suggested.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- Amino Sugars
- metabolism
- Antigens
- CD
- Antimicrobial Cationic Peptides
- Carbohydrate Metabolism
- Carbohydrate Sequence
- Electrophoresis
- Polyacrylamide Gel
- Lactosylceramides
- metabolism
- Lectins
- chemistry
- isolation & purification
- metabolism
- Magnetic Resonance Spectroscopy
- Models
- Molecular
- Molecular Sequence Data
- Plant Lectins
- Plant Proteins
- chemistry
- Sequence Alignment
- Solanum tuberosum
- chemistry
- Structure-Activity Relationship
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Ciopraga, J
-
Ångström, Jonas, ...
-
Bergström, Jörge ...
-
Larsson, Thomas, ...
-
Karlsson, Niclas ...
-
Motas, C
-
show more...
-
Gozia, O
-
Teneberg, Susann ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Other Basic Medi ...
- Articles in the publication
-
Journal of bioch ...
- By the university
-
University of Gothenburg