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Analysis of Amino-T...
Analysis of Amino-Terminal Variants of Amyloid-β Peptides by Capillary Isoelectric Focusing Immunoassay.
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Haußmann, Ute (author)
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Jahn, Olaf (author)
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Linning, Philipp (author)
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Janßen, Christin (author)
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Liepold, Thomas (author)
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- Portelius, Erik, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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- Zetterberg, Henrik, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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Bauer, Chris (author)
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Schuchhardt, Johannes (author)
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Knölker, Hans-Joachim (author)
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Klafki, Hans (author)
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Wiltfang, Jens (author)
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(creator_code:org_t)
- 2013-08-14
- 2013
- English.
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In: Analytical chemistry. - : American Chemical Society (ACS). - 1520-6882 .- 0003-2700. ; 85:17, s. 8142-9
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Abstract
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- Here we present a novel assay for the separation and detection of amino-terminal amyloid-β (Aβ) peptide variants by capillary isoelectric focusing (CIEF) immunoassay. Specific amino-terminally truncated Aβ peptides appear to be generated by β-secretase (BACE1)-independent mechanisms and have previously been observed in cerebrospinal fluid (CSF) after BACE1 inhibitor treatment in an animal model. CIEF immunoassay sensitivity is sufficient to detect total Aβ in CSF without preconcentration. To analyze low-abundance amino-terminally truncated Aβ peptides from cell culture supernatants, we developed a CIEF-compatible immunoprecipitation protocol, allowing for selective elution of Aβ peptides with very low background. CIEF immunoassay and immunoprecipitation mass spectrometry analysis identified peptides starting at residue Arg(5) as the main amino-terminal Aβ variants produced in the presence of tripartite BACE1 inhibitor in our cell culture model. The CIEF immunoassay allows for robust relative quantification of Aβ peptide patterns in biological samples. To assess the future possibility of absolute quantification, we have prepared the Aβ peptides Aβx-10, Aβx-16, and Aβ5-38(D23S) by using solid phase peptide synthesis as internal standards for the CIEF immunoassay.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Haußmann, Ute
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Jahn, Olaf
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Linning, Philipp
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Janßen, Christin
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Liepold, Thomas
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Portelius, Erik, ...
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show more...
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Zetterberg, Henr ...
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Bauer, Chris
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Schuchhardt, Joh ...
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Knölker, Hans-Jo ...
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Klafki, Hans
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Wiltfang, Jens
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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Analytical chemi ...
- By the university
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University of Gothenburg