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Human and Methodolo...
Human and Methodological Sources of Variability in the Measurement of Urinary 8-Oxo-7,8-dihydro-2 '-deoxyguanosine
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- Barregård, Lars, 1948 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine
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Moller, P. (author)
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Henriksen, T. (author)
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Mistry, V. (author)
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Koppen, G. (author)
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Rossner, P. (author)
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Sram, R. J. (author)
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Weimann, A. (author)
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Poulsen, H. E. (author)
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Nataf, R. (author)
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Andreoli, R. (author)
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Manini, P. (author)
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Marczylo, T. (author)
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Lam, P. (author)
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Evans, M. D. (author)
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Kasai, H. (author)
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- Kawai, K. (author)
- Karolinska Institutet
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Li, Y. S. (author)
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Sakai, K. (author)
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Singh, R. (author)
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Teichert, F. (author)
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Farmer, P. B. (author)
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Rozalski, R. (author)
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Gackowski, D. (author)
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Siomek, A. (author)
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Saez, G. T. (author)
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Cerda, C. (author)
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- Broberg Palmgren, Karin (author)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
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- Lindh, Christian (author)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
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- Hossain, Bakhtiar (author)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
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- Haghdoost, Siamak (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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Hu, C. W. (author)
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Chao, M. R. (author)
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Wu, K. Y. (author)
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Orhan, H. (author)
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Senduran, N. (author)
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Smith, R. J. (author)
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Santella, R. M. (author)
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Su, Y. L. (author)
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Cortez, C. (author)
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Yeh, S. (author)
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Olinski, R. (author)
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Loft, S. (author)
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Cooke, M. S. (author)
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2013
- 2013
- English.
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In: Antioxidants and Redox Signaling. - : Mary Ann Liebert Inc. - 1523-0864 .- 1557-7716. ; 18:18, s. 2377-2391
- Related links:
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https://europepmc.or...
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http://dx.doi.org/10...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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http://kipublication...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Aims: Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability. Results: Chromatographic assays showed high agreement across urines from different subjects, whereas ELISAs showed far more inter-laboratory variation and generally overestimated levels, compared to the chromatographic assays. Excretion rates in timed 'spot' samples showed strong correlations with 24 h excretion (the 'gold' standard) of urinary 8-oxodG (r(p) 0.67-0.90), although the associations were weaker for 8-oxodG adjusted for creatinine or specific gravity (SG). The within-individual excretion of 8-oxodG varied only moderately between days (CV 17% for 24 h excretion and 20% for first void, creatinine-corrected samples). Innovation: This is the first comprehensive study of both human and methodological factors influencing 8-oxodG measurement, providing key information for future studies with this important biomarker. Conclusion: ELISA variability is greater than chromatographic assay variability, and cannot determine absolute levels of 8-oxodG. Use of standardized calibrants greatly improves intra-technique agreement and, for the chromatographic assays, importantly allows integration of results for pooled analyses. If 24 h samples are not feasible, creatinine- or SG-adjusted first morning samples are recommended.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- performance liquid-chromatography
- tandem mass-spectrometry
- oxidative
- stress
- automated-method
- in-vivo
- dna
- excretion
- damage
- 8-hydroxy-2'-deoxyguanosine
- 8-hydroxydeoxyguanosine
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Barregård, Lars, ...
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Moller, P.
-
Henriksen, T.
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Mistry, V.
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Koppen, G.
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Rossner, P.
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show more...
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Sram, R. J.
-
Weimann, A.
-
Poulsen, H. E.
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Nataf, R.
-
Andreoli, R.
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Manini, P.
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Marczylo, T.
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Lam, P.
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Evans, M. D.
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Kasai, H.
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Kawai, K.
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Li, Y. S.
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Sakai, K.
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Singh, R.
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Teichert, F.
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Farmer, P. B.
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Rozalski, R.
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Gackowski, D.
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Siomek, A.
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Saez, G. T.
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Cerda, C.
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Broberg Palmgren ...
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Lindh, Christian
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Hossain, Bakhtia ...
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Haghdoost, Siama ...
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Hu, C. W.
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Chao, M. R.
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Wu, K. Y.
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Orhan, H.
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Senduran, N.
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Smith, R. J.
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Santella, R. M.
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Su, Y. L.
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Cortez, C.
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Yeh, S.
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Olinski, R.
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Loft, S.
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Cooke, M. S.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomedical Labor ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Antioxidants and ...
- By the university
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University of Gothenburg
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Lund University
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Karolinska Institutet
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Stockholm University