Reduced albumin extravasation in experimental rat skin and muscle burn injury by D-myo-inositol-1,2,6-trisphosphate treatment.

• This study investigated the effects of the anti-inflammatory agent D-myo-inositol-1,2,6-trisphosphate (IP3) on burn edema. Two sets of experiments were performed. In the first set, a full-thickness burn injury was induced in the abdominal skin of anesthetized rats. Postburn intravenous treatment was given with IP3, indomethacin or saline solution. Extravasation of Evans blue albumin in the burned tissue was quantified by a spectrophotometric technique. Results showed significant inhibition of albumin extravasation by IP3 in three of five different doses compared to saline-treated animals. In the second set of experiments, a deep full-thickness burn through the abdominal skin and rectus muscle was induced. The therapeutic window of IP3 could be more well-defined. Resulted showed a significant reduction of albumin extravasation in the skin at all four dose levels and in the abdominal muscle at three of four doses. Indomethacin had no significant effect on postburn edema formation. The mechanisms responsible for the inhibition of albumin leakage by IP3 could be secondary to reduced formation of edema-promoting inflammatory mediators by the agent, resulting in improved vascular patency.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Kirurgi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Surgery (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Anestesi och intensivvård (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Anesthesiology and Intensive Care (hsv//eng)

Keyword

Albumins

metabolism

Analysis of Variance

Animals

Anti-Inflammatory Agents

Non-Steroidal

administration & dosage

therapeutic use

Burns

drug therapy

metabolism

pathology

Confidence Intervals

Disease Models

Animal

Dose-Response Relationship

Drug

Edema

drug therapy

etiology

pathology

Evans Blue

diagnostic use

Extravasation of Diagnostic and Therapeutic Materials

Indomethacin

administration & dosage

therapeutic use

Inositol Phosphates

administration & dosage

therapeutic use

Muscles

drug effects

metabolism

pathology

Rats

Rats

Sprague-Dawley

Skin

drug effects

metabolism

pathology

Sodium Chloride

administration & dosage

therapeutic use

Publication and Content Type

ref (subject category)

art (subject category)