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Diversity of human ...
Diversity of human papillomaviruses in skin lesions
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- Ekström, Johanna (author)
- Lund University,Lunds universitet,Internmedicin - epidemiologi,Forskargrupper vid Lunds universitet,Klinisk mikrobiologi, Malmö,Internal Medicine - Epidemiology,Lund University Research Groups,Clinical Microbiology, Malmö
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- Söderlund Strand, Anna (author)
- Karolinska Institutet,Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups
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- Bzhalava, Davit (author)
- Karolinska Institutet,Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups
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Soderlund-Strand, A. (author)
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- Hultin, E. (author)
- Karolinska Institutet
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Nordin, P. (author)
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- Stenquist, Bo, 1937 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för dermatologi och venereologi,Institute of Clinical Sciences, Department of Dermatology and Venereology
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- Paoli, John, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för dermatologi och venereologi,Institute of Clinical Sciences, Department of Dermatology and Venereology
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- Forslund, Ola (author)
- Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups
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- Dillner, Joakim (author)
- Karolinska Institutet,Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups
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(creator_code:org_t)
- Elsevier BV, 2013
- 2013
- English.
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In: Virology. - : Elsevier BV. - 0042-6822 .- 1096-0341. ; 447:1-2, s. 300-311
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Abstract
Subject headings
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- Pools of frozen biopsies from patients with squamous cell carcinoma (SCC) (n=29) actinic keratosis (AK) (n=31), keratoacanthoma (n=91) and swab samples from 84 SCCs and 91 AKs were analysed with an extended HPV general primer PCR and high-throughput sequencing of amplimers. We found 273 different HPV isolates (87 known HPV types, 139 previously known HPV sequences (putative types) and 47 sequences from novel putative HPV types). Among the new sequences, five clustered in genus Betapapillomavirus and 42 in genus Gammapapillomavirus. Resequencing of the three pools between 21 to 70 times resulted in the detection of 283 different known or putative HPV types, with 156 different sequences found in only one of the pools. Type-specific PCRs for 37 putative types from an additional 296 patients found only two of these putative types. In conclusion, skin lesions contain a large diversity of HPV types, but most appeared to be rare infections. (C) 2013 Elsevier Inc. All rights reserved.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- Human papillomaviruses
- Skin lesions
- SQUAMOUS-CELL CARCINOMA
- CUTANEOUS HUMAN PAPILLOMAVIRUSES
- EPIDERMODYSPLASIA-VERRUCIFORMIS
- TRANSPLANT RECIPIENTS
- CERVICAL-CANCER
- NESTED PCR
- HPV TYPES
- DNA
- INDIVIDUALS
- SEQUENCES
- RSLUND O
- 1994
- JOURNAL OF VIROLOGICAL METHODS
- V49
- P129
Publication and Content Type
- ref (subject category)
- art (subject category)
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Virology
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- By the author/editor
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Ekström, Johanna
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Söderlund Strand ...
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Bzhalava, Davit
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Soderlund-Strand ...
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Hultin, E.
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Nordin, P.
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Stenquist, Bo, 1 ...
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Paoli, John, 197 ...
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Forslund, Ola
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Dillner, Joakim
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Dermatology and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
- Articles in the publication
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Virology
- By the university
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University of Gothenburg
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Lund University
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Karolinska Institutet