Search: onr:"swepub:oai:gup.ub.gu.se/193899" >
Differential regula...
Differential regulation of mesothelial cell fibrinolysis by transforming growth factor beta 1.
-
- Falk, Peter, 1962 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna,Institute of Surgical Sciences
-
Ma, C (author)
-
Chegini, N (author)
-
show more...
-
- Holmdahl, Lena, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
-
show less...
-
(creator_code:org_t)
- 2000
- 2000
- English.
-
In: Scandinavian journal of clinical and laboratory investigation. - 0036-5513. ; 60:6, s. 439-47
- Related links:
-
https://gup.ub.gu.se...
Abstract
Subject headings
Close
- Inflammation and tissue trauma during the surgical procedure reduce the peritoneal fibrinolytic capacity. These conditions promote adhesion formation, and are associated with increased expression of transforming growth factor beta 1 (TGF-beta1). The objective of the present study was to investigate whether TGF-beta1 regulates the expression of fibrinolytic components in peritoneal mesothelial cells. Human peritoneal mesothelial cells (HPMC) were cultured and treated with various concentrations of human recombinant TGF-beta1 (0.1, 1.0 and 10 ng/mL) for 24 h. Levels of tissue- and urokinase plasminogen activator (t-PA and uPA), plasminogen activator inhibitor type-1 (PAI-1) and type-2 (PAI-2) mRNA and protein were assessed by quantitative reverse transcriptase polymerase chain reaction (Q-RT-PCR) and ELISA, respectively. HPMC expressed these components at the gene and protein level. TGF-beta1 downregulated, dose-dependently t-PA mRNA and protein to about 50% of control values (p = 0.0010), and doubled PAI-1 protein production (p = 0.0008) compared to untreated controls. Although uPA gene expression increased in cells exposed to TGF-beta1, the corresponding protein concentration in conditioned media did not. PAI-2 was not affected, either at the gene or protein level. In conclusion, the results indicate that fibrinolytic capacity of mesothelial cells is reduced by TGF-beta1, suggesting that peritoneal adhesion formation induced by TGF-beta1 may be mediated, in part, through reduction in fibrin degradation capacity at an early stage of peritoneal tissue repair.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Enzyme-Linked Immunosorbent Assay
- Epithelial Cells
- drug effects
- Fibrinolysis
- genetics
- Gene Expression Profiling
- Humans
- Peritoneal Cavity
- pathology
- physiopathology
- Plasminogen Activator Inhibitor 1
- genetics
- Plasminogen Activator Inhibitor 2
- genetics
- RNA
- Messenger
- genetics
- metabolism
- Receptors
- Cell Surface
- genetics
- Receptors
- Urokinase Plasminogen Activator
- Recombinant Proteins
- pharmacology
- Reverse Transcriptase Polymerase Chain Reaction
- Tissue Plasminogen Activator
- genetics
- Transforming Growth Factor beta
- pharmacology
- Urokinase-Type Plasminogen Activator
- genetics
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database