Search: onr:"swepub:oai:gup.ub.gu.se/198744" >
Predicting the viru...
Predicting the virulence of MRSA from its genome sequence
-
Laabei, M. (author)
-
Recker, M. (author)
-
Rudkin, J. K. (author)
-
show more...
-
Aldeljawi, M. (author)
-
Gulay, Z. (author)
-
Sloan, T. J. (author)
-
Williams, P. (author)
-
Endres, J. L. (author)
-
Bayles, K. W. (author)
-
Fey, P. D. (author)
-
Yajjala, V. K. (author)
-
Widhelm, T. (author)
-
Hawkins, E. (author)
-
Lewis, K. (author)
-
Parfett, S. (author)
-
Scowen, L. (author)
-
Peacock, S. J. (author)
-
Holden, M. (author)
-
Wilson, D. (author)
-
Read, T. D. (author)
-
van den Elsen, J. (author)
-
Priest, N. K. (author)
-
Feil, E. J. (author)
-
Hurst, L. D. (author)
-
- Josefsson, Elisabet, 1966 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
-
Massey, R. C. (author)
-
show less...
-
(creator_code:org_t)
- 2014-04-09
- 2014
- English.
-
In: Genome Research. - : Cold Spring Harbor Laboratory. - 1088-9051. ; 24:5, s. 839-849
- Related links:
-
https://gup.ub.gu.se... (primary) (free)
-
show more...
-
http://genome.cshlp....
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Microbial virulence is a complex and often multifactorial phenotype, intricately linked to a pathogen's evolutionary trajectory. Toxicity, the ability to destroy host cell membranes, and adhesion, the ability to adhere to human tissues, are the major virulence factors of many bacterial pathogens, including Staphylococcus aureus. Here, we assayed the toxicity and adhesiveness of 90 MRSA (methicillin resistant S. aureus) isolates and found that while there was remarkably little variation in adhesion, toxicity varied by over an order of magnitude between isolates, suggesting different evolutionary selection pressures acting on these two traits. We performed a genome-wide association study (GWAS) and identified a large number of loci, as well as a putative network of epistatically interacting loci, that significantly associated with toxicity. Despite this apparent complexity in toxicity regulation, a predictive model based on a set of significant single nucleotide polymorphisms (SNPs) and insertion and deletions events (indels) showed a high degree of accuracy in predicting an isolate's toxicity solely from the genetic signature at these sites. Our results thus highlight the potential of using sequence data to determine clinically relevant parameters and have further implications for understanding the microbial virulence of this opportunistic pathogen.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Keyword
- RESISTANT STAPHYLOCOCCUS-AUREUS
- MYCOBACTERIUM-TUBERCULOSIS
- EVOLUTION
- IDENTIFICATION
- EXPRESSION
- GENE
- BIOSYNTHESIS
- MICROBIOLOGY
- TRANSMISSION
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Laabei, M.
-
Recker, M.
-
Rudkin, J. K.
-
Aldeljawi, M.
-
Gulay, Z.
-
Sloan, T. J.
-
show more...
-
Williams, P.
-
Endres, J. L.
-
Bayles, K. W.
-
Fey, P. D.
-
Yajjala, V. K.
-
Widhelm, T.
-
Hawkins, E.
-
Lewis, K.
-
Parfett, S.
-
Scowen, L.
-
Peacock, S. J.
-
Holden, M.
-
Wilson, D.
-
Read, T. D.
-
van den Elsen, J ...
-
Priest, N. K.
-
Feil, E. J.
-
Hurst, L. D.
-
Josefsson, Elisa ...
-
Massey, R. C.
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Microbiology in ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Medical Genetics
- Articles in the publication
-
Genome Research
- By the university
-
University of Gothenburg