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A new approach to m...
A new approach to measuring vitamin D in human adipose tissue using time-of-flight secondary ion mass spectrometry: A pilot study
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- Malmberg, Per, 1974 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Karlsson, Therese, 1979 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology,University of Gothenburg
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- Svensson, Henrik, 1982 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,University of Gothenburg
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- Lönn, Malin, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,University of Gothenburg
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- Carlsson, Nils-Gunnar, 1951 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Sandberg, Ann-Sofie, 1951 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Jennische, Eva, 1949 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,University of Gothenburg
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- Osmancevic, Amra, 1968 (author)
- Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital
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- Holmäng, Agneta, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology,University of Gothenburg
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(creator_code:org_t)
- Elsevier BV, 2014
- 2014
- English.
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In: Journal of Photochemistry and Photobiology. B: Biology. - : Elsevier BV. - 1011-1344. ; 138, s. 295-301
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Abstract
Subject headings
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- Circulating concentrations of vitamin D, 25(OH)D, and 1,25(OH)2D are lower in obese than lean individuals, but little is known about the adipose tissue content of these molecules. The aim of this study was to explore the possibility to use time-of-flight secondary ion mass spectrometry (TOF-SIMS) to measure vitamin D and its metabolites in fat tissue in obese and lean subjects. Abdominal subcutaneous adipose tissue (SAT) biopsies were obtained from three lean and three obese women, and paired biopsies SAT and visceral adipose tissue (VAT) were obtained from three obese subjects during gastric bypass surgery. TOF-SIMS was used to measure vitamin D3, 25(OH)D3, and 1,25(OH)2D3 in adipose tissue. We found that vitamin D3, 25(OH)D3, and 1,25(OH)2D3 in adipose tissue can be measured with TOF-SIMS. In adipose tissue, vitamin D3 and its metabolites were located in adipocyte lipid droplets. The content of vitamin D3 (P = 0.006) and 25(OH)D3 (P = 0.018) were lower in SAT in obese compared with lean women. TOF-SIMS has the potential to semi-quantitatively measure vitamin D metabolites in adipose tissue, and offers a possibility to compare vitamin D levels in different depots and groups of individuals. It also gives the opportunity to explore the localization of vitamin D metabolites at a cellular level.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Keyword
- Adipose tissue
- Vitamin D
- TOF-SIMS
- Vitamin D
Publication and Content Type
- ref (subject category)
- art (subject category)
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Malmberg, Per, 1 ...
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Karlsson, Theres ...
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Svensson, Henrik ...
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Lönn, Malin, 195 ...
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Carlsson, Nils-G ...
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Sandberg, Ann-So ...
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Jennische, Eva, ...
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Osmancevic, Amra ...
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Holmäng, Agneta, ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Dermatology and ...
- Articles in the publication
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Journal of Photo ...
- By the university
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University of Gothenburg
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Chalmers University of Technology