ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes

• The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleate in cultured cells. Also, that addition of cyclic AMP shifts ARFGAP1 from lipid droplets to the Golgi apparatus and that overexpression and knockdown of ARFGAP1 affect lipid droplet formation. Examination of human liver tissue reveals that ARFGAP1 is found associated with lipid droplets at steady state in some but not all hepatocytes.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)

Keyword

ADP-RIBOSYLATION FACTOR

GTPASE-ACTIVATING PROTEIN

GOLGI-COMPLEX

COPI

VESICLES

ENDOPLASMIC-RETICULUM

BINDING PROTEINS

COATED VESICLES

PHOSPHOLIPASE-D

BETA-COP

ARF

Multidisciplinary Sciences

ATES OF AMERICA

V110

P13244

Publication and Content Type

ref (subject category)

art (subject category)