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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02676naa a2200445 4500 | |
| 001 | oai:gup.ub.gu.se/211807 | |
| 003 | SwePub | |
| 008 | 240528s1990 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://gup.ub.gu.se/publication/2118072 URI |
| 040 | a (SwePub)gu | |
| 041 | a eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Holgersson, J4 aut |
| 245 | 1 0 | a Blood group A glycolipid antigen biosynthesis: discrimination between biosynthesized and enzyme preparation derived blood group A antigen by mass spectrometry. |
| 264 | 1 | c 1990 |
| 520 | a A monofucosyl type 1 chain blood group A hexaglycosylceramide was biosynthesized in solution using the type 1 chain blood group H pentaglycosylceramide as precursor, a crude microsomal fraction prepared from the mucosa scraping of a blood group A pig small intestine as enzyme source, and uridine diphosphate-N-acetyl-(1-14C)galactosamine as sugar donor. The radioactive product was enriched using reversed-phase column chromatography and silica gel HPLC. The peak, as detected by a beta-flow scintillation counter, was collected, permethylated, and analyzed by mass spectrometry. Carbohydrate sequence ions were found, indicating the presence of both the biosynthesized and a native, non-14C-containing blood group A hexaglycosylceramide. The blood group A pig small intestinal mucosa used as the enzyme source contain blood group A hexaglycosylceramide as the predominant glycolipid. Therefore, it is concluded that the nonradioactive blood group A hexaglycosylceramide found after the biosynthesis is derived from the enzyme preparation. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicin0 (SwePub)3022 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicine0 (SwePub)3022 hsv//eng |
| 653 | a ABO Blood-Group System | |
| 653 | a Animals | |
| 653 | a Autoradiography | |
| 653 | a Chromatography | |
| 653 | a High Pressure Liquid | |
| 653 | a Chromatography | |
| 653 | a Thin Layer | |
| 653 | a Glycolipids | |
| 653 | a isolation & purification | |
| 653 | a Mass Spectrometry | |
| 653 | a Methylation | |
| 653 | a Silica Gel | |
| 653 | a Silicon Dioxide | |
| 653 | a Swine | |
| 700 | 1 | a Jacobsson, A4 aut |
| 700 | 1 | a Breimer, Michael,d 1951u Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine4 aut0 (Swepub:gu)xbremi |
| 700 | 1 | a Samuelsson, B E4 aut |
| 710 | 2 | a Göteborgs universitetb Medicinska institutionen4 org |
| 773 | 0 | t Analytical biochemistryg 184:1, s. 145-50q 184:1<145-50x 0003-2697 |
| 856 | 4 8 | u https://gup.ub.gu.se/publication/211807 |
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