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Glycolipid- and gly...
Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.
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Holgersson, J (author)
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- Breimer, Michael, 1951 (author)
- Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine
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Jacobsson, A (author)
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Svensson, L (author)
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Ulfvin, A (author)
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Samuelsson, B E (author)
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(creator_code:org_t)
- 1990
- 1990
- English.
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In: Glycoconjugate journal. - 0282-0080. ; 7:6, s. 601-8
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- Total non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Keyword
- ABO Blood-Group System
- blood
- immunology
- Blood Component Transfusion
- Blood Platelets
- metabolism
- Chromatography
- Thin Layer
- Glycolipids
- blood
- Glycoproteins
- blood
- Humans
- Isoantigens
- biosynthesis
- blood
- metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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