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Lipidic cubic phase...
Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
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Nogly, P. (author)
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James, D. (author)
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Wang, D. J. (author)
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White, T. A. (author)
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Zatsepin, N. (author)
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Shilova, A. (author)
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Nelson, G. (author)
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Liu, H. G. (author)
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Johansson, L. (author)
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Heymann, M. (author)
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Jaeger, K. (author)
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Metz, M. (author)
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Wickstrand, C. (author)
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Wu, W. T. (author)
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Bath, P. (author)
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- Berntsen, Peter, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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Oberthuer, D. (author)
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Panneels, V. (author)
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Cherezov, V. (author)
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Chapman, H. (author)
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Schertler, G. (author)
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- Neutze, Richard, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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Spence, J. (author)
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Moraes, I. (author)
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Burghammer, M. (author)
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Standfuss, J. (author)
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Weierstall, U. (author)
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(creator_code:org_t)
- 2015-03-01
- 2015
- English.
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In: Iucrj. - : International Union of Crystallography (IUCr). - 2052-2525. ; 2
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http://dx.doi.org/10...
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Abstract
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- Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven proton-pump bacteriorhodopsin (bR) at a resolution of 2.4 angstrom. The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- lipidic cubic phases
- protein crystallography
- bacteriorhodopsin
- XFEL
- PROTEIN-STRUCTURE DETERMINATION
- X-RAY CRYSTALLOGRAPHY
- FREE-ELECTRON
- LASER
- FEMTOSECOND CRYSTALLOGRAPHY
- MEMBRANE-PROTEINS
- MACROMOLECULAR
- CRYSTALLOGRAPHY
- CRYSTALLIZATION
- DAMAGE
- RESOLUTION
- SOFTWARE
- Chemistry
- Multidisciplinary
- Crystallography
- Materials Science
- Multidisciplinary
- protein crystallography
Publication and Content Type
- ref (subject category)
- art (subject category)
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Iucrj
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To the university's database
- By the author/editor
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Nogly, P.
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James, D.
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Wang, D. J.
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White, T. A.
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Zatsepin, N.
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Shilova, A.
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show more...
-
Nelson, G.
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Liu, H. G.
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Johansson, L.
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Heymann, M.
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Jaeger, K.
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Metz, M.
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Wickstrand, C.
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Wu, W. T.
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Bath, P.
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Berntsen, Peter, ...
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Oberthuer, D.
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Panneels, V.
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Cherezov, V.
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Chapman, H.
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Schertler, G.
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Neutze, Richard, ...
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Spence, J.
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Moraes, I.
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Burghammer, M.
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Standfuss, J.
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Weierstall, U.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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Iucrj
- By the university
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University of Gothenburg
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Chalmers University of Technology