Imaging Mass Spectrometry for Single-Cell Analysis

Phan, Nhu TN, 1981 (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg

Fletcher, John S. (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg

Ewing, Andrew G, 1957 (author): Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

ISBN 9780124095472
Elsevier, 2014
2014
English.
In: Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. - : Elsevier. - 9780124095472

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Abstract Subject headings

Single-cell imaging is a very important area in biological, medical, and pharmaceutical research, yet it is one of the most challenging fields for mass spectrometry. Imaging mass spectrometry (IMS), which is a powerful label-free analytical technique, has proved its capability to chemically visualize single cells with high sensitivity, chemical selectivity, and in certain cases subcellular spatial resolution. We present here an overview of the capabilities and current progress of IMS, particularly secondary ion mass spectrometry, matrix-assisted laser desorption ionization, and desorption electrospray ionization for single-cell imaging. The principles and technical developments of each technique are introduced. Critical aspects regarding single-cell imaging are addressed, especially sensitivity, spatial resolution, and sample handling. Current achievements in this field of application are also presented for both 2-D imaging and 3-D imaging. Furthermore, we address the potential contribution of IMS single-cell ‘omics’ and discuss its future development.

By the author/editor: Phan, Nhu TN, 19 ...; Fletcher, John S ...; Ewing, Andrew G, ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Analytical Chemi ...

By the university: University of Gothenburg; Chalmers University of Technology

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