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The oral microbiome...
The oral microbiome in human immunodeficiency virus (HIV)-positive individuals
- Article/chapterEnglish2015
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Microbiology Society,2015
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LIBRIS-ID:oai:gup.ub.gu.se/226082
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https://gup.ub.gu.se/publication/226082URI
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https://doi.org/10.1099/jmm.0.000128DOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Human immunodeficiency virus (HIV) infection is associated with a range of oral conditions, and increased numbers of disease-associated microbial species have previously been found in HIV-positive subjects. The aim of this study was to use next-generation sequencing to compare the composition of the oral microbiome in HIV-positive and -negative individuals. Plaque and saliva were collected from 37 HIV-positive individuals and 37 HIV-negative individuals, and their bacterial composition determined by pyrosequencing of partial 16S rRNA genes. A total of 855 222 sequences were analysed. The number of species-level operational taxonomic units (OTUs) detected was significantly lower in the saliva of HIV-positive individuals (mean=303.3) than in that of HIV-negative individuals (mean=365.5) (P<0.0003). Principal coordinates analysis (PCoA) based on community membership (Jaccard index) and structure (Yue and Clayton measure of dissimilarity) showed significant separation of plaque and saliva samples [analysis of molecular variance (AMOVA), P<0.001]. PCoA plots did not show any clear separation based on HIV status. However, AMOVA indicated that there was a significant difference in the community membership of saliva between HIV-positive and -negative groups (P=0.001). Linear discriminant analysis effect size revealed an OTU identified as Haemophilus parainfluenzae to be significantly associated with HIV-positive individuals, whilst Streptococcus mitis/HOT473 was most significantly associated with HIV-negative individuals. In conclusion, this study has confirmed that the microbial composition of saliva and plaque is different. The oral microbiomes of HIV-positive and -negative individuals were found to be similar overall, although there were minor but significant differences in the composition of the salivary microbiota of the two groups.
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Arirachakaran, P.
(author)
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Poovorawan, Y.
(author)
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Dahlén, Gunnar,1944Gothenburg University,Göteborgs universitet,Institutionen för odontologi, sektion 3,Institute of Odontology, Section 3(Swepub:gu)xdahgu
(author)
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Wade, W. G.
(author)
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Göteborgs universitetInstitutionen för odontologi, sektion 3
(creator_code:org_t)
Related titles
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In:Journal of Medical Microbiology: Microbiology Society64:Part: 9, s. 1094-11010022-26151473-5644
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