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Regulated assembly ...
Regulated assembly of a supramolecular centrosome scaffold in vitro
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Woodruff, J. B. (author)
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Wueseke, O. (author)
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Viscardi, V. (author)
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Mahamid, J. (author)
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Ochoa, S. D. (author)
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Bunkenborg, J. (author)
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- Widlund, Per O (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Pozniakovsky, A. (author)
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Zanin, E. (author)
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Bahmanyar, S. (author)
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Zinke, A. (author)
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Hong, S. H. (author)
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Decker, M. (author)
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Baumeister, W. (author)
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Andersen, J. S. (author)
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Oegema, K. (author)
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Hyman, A. A. (author)
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(creator_code:org_t)
- 2015-05-14
- 2015
- English.
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In: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 348:6236, s. 808-812
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Abstract
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- The centrosome organizes microtubule arrays within animal cells and comprises two centrioles surrounded by an amorphous protein mass called the pericentriolar material (PCM). Despite the importance of centrosomes as microtubule-organizing centers, the mechanism and regulation of PCM assembly are not well understood. In Caenorhabditis elegans, PCM assembly requires the coiled-coil protein SPD-5.We found that recombinant SPD-5 could polymerize to form micrometer-sized porous networks in vitro. Network assembly was accelerated by two conserved regulators that control PCM assembly in vivo, Polo-like kinase-1 and SPD-2/Cep192. Only the assembled SPD-5 networks, and not unassembled SPD-5 protein, functioned as a scaffold for other PCM proteins. Thus, PCM size and binding capacity emerge from the regulated polymerization of one coiled-coil protein to form a porous network.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Woodruff, J. B.
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Wueseke, O.
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Viscardi, V.
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Mahamid, J.
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Ochoa, S. D.
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Bunkenborg, J.
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show more...
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Widlund, Per O
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Pozniakovsky, A.
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Zanin, E.
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Bahmanyar, S.
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Zinke, A.
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Hong, S. H.
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Decker, M.
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Baumeister, W.
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Andersen, J. S.
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Oegema, K.
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Hyman, A. A.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Other Basic Medi ...
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Science
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University of Gothenburg