Search: onr:"swepub:oai:gup.ub.gu.se/230019" >
Expression, sorting...
Expression, sorting, and segregation of Golgi proteins during germ cell differentiation in the testis
-
Au, C. E. (author)
-
Hermo, L. (author)
-
Byrne, E. (author)
-
show more...
-
Smirle, J. (author)
-
Fazel, A. (author)
-
Simon, P. H. G. (author)
-
Kearney, R. E. (author)
-
Cameron, P. H. (author)
-
Smith, C. E. (author)
-
Vali, H. (author)
-
- Fernandez-Rodriguez, Julia, 1965 (author)
- Gothenburg University,Göteborgs universitet,Core Facilities, Centre for Cellular Imaging,Core Facilities, Centre for Cellular Imaging
-
Ma, K. W. (author)
-
Nilsson, T. (author)
-
Bergeron, J. J. M. (author)
-
show less...
-
(creator_code:org_t)
- 2015
- 2015
- English.
-
In: Molecular Biology of the Cell. - 1059-1524. ; 26:22, s. 4015-4032
- Related links:
-
https://gup.ub.gu.se...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- The molecular basis of changes in structure, cellular location, and function of the Golgi apparatus during male germ cell differentiation is unknown. To deduce cognate Golgi proteins, we isolated germ cell Golgi fractions, and 1318 proteins were characterized, with 20 localized in situ. The most abundant protein, GL54D of unknown function, is characterized as a germ cell-specific Golgi-localized type II integral membrane glycoprotein. TM9SF3, also of unknown function, was revealed to be a universal Golgi marker for both somatic and germ cells. During acrosome formation, several Golgi proteins (GBF1, GPP34, GRASP55) localize to both the acrosome and Golgi, while GL54D, TM9SF3, and the Golgi trafficking protein TMED7/p27 are segregated from the acrosome. After acrosome formation, GL54D, TM9SF3, TMED4/p25, and TMED7/p27 continue to mark Golgi identity as it migrates away from the acrosome, while the others (GBF1, GPP34, GRASP55) remain in the acrosome and are progressively lost in later steps of differentiation. Cytoplasmic HSP70.2 and the endoplasmic reticulum luminal protein-folding enzyme PDILT are also Golgi recruited but only during acrosome formation. This resource identifies abundant Golgi proteins that are expressed differentially during mitosis, meiosis, and postacrosome Golgi migration, including the last step of differentiation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- MANNOSE 6-PHOSPHATE RECEPTOR
- CLATHRIN-COATED VESICLES
- IN-VIVO ROLE
- ENDOPLASMIC-RETICULUM
- PLASMA-MEMBRANE
- N-GLYCANS
- 3-DIMENSIONAL
- ARCHITECTURE
- MAMMALIAN SPERMATOGENESIS
- TARGETED DISRUPTION
- CYTOPLASMIC DROPLET
- Cell Biology
- ATES OF AMERICA
- V104
- P8346
- NG XM
- 1982
- AMERICAN JOURNAL OF ANATOMY
- V163
- P283
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Au, C. E.
-
Hermo, L.
-
Byrne, E.
-
Smirle, J.
-
Fazel, A.
-
Simon, P. H. G.
-
show more...
-
Kearney, R. E.
-
Cameron, P. H.
-
Smith, C. E.
-
Vali, H.
-
Fernandez-Rodrig ...
-
Ma, K. W.
-
Nilsson, T.
-
Bergeron, J. J. ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Molecular Biolog ...
- By the university
-
University of Gothenburg