Nested PCR for detection of HSV-1 in oral mucosa

Jalouli, Jamshid (author): Uppsala universitet,Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology,Käkkirurgi

Hasséus, Bengt, 1955 (author): Gothenburg University,Göteborgs universitet,Institutionen för odontologi, sektion 1,Institute of Odontology, Section 1

Öhman, J. (author)

(creator_code:org_t)

Medicina Oral, S.L. 2015
2015
English.
In: Medicina Oral, Patologia Oral y Cirugia Bucal. - : Medicina Oral, S.L.. - 1698-4447 .- 1698-6946. ; 20:6

Related links:: https://doi.org/10.4...; show more...; https://gup.ub.gu.se...; https://doi.org/10.4...; https://urn.kb.se/re...; show less...

Abstract Subject headings

© Medicina Oral S. L. Background: It has been estimated that 15%-20% of human tumours are driven by infection and inflammation, and viral infections play an important role in malignant transformation. The evidence that herpes simplex virus type 1 (HSV-1) could be involved in the aetiology of oral cancer varies from weak to persuasive. This study aimed to investigate by nested PCR (NPCR) the prevalence of HSV-1 in samples from normal oral mucosa, oral leukoplakia, and oral squamous cell carcinoma (OSCC). Material and Methods: We investigated the prevalence of HSV-1 in biopsies obtained from 26 fresh, normal oral mucosa from healthy volunteers as well as 53 oral leukoplakia and 27 OSCC paraffin-embedded samples. DNA was extracted from the specimens and investigated for the presence of HSV-1 by nested polymerase chain reaction (NPCR) and DNA sequencing. Results: HSV-1 was detected in 14 (54%) of the healthy samples, in 19 (36%) of the oral leukoplakia samples, and in 14 (52%) of the OSCC samples. The differences were not statistically significant. Conclusions: We observed a high incidence of HSV-1 in healthy oral mucosa, oral leukoplakia, and OSCC tissues. Thus, no connection between OSCC development and presence of HSV-1 was detected.

By the author/editor: Jalouli, Miranda; Jalouli, Jamshid; Hasséus, Bengt, ...; Öhman, J.; Hirsch, Jan M; Sand, Lars

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Dentistry

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