Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels

Westerlund, Fredrik,1978Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),Chalmers tekniska högskola,Chalmers University of Technology,University of Gothenburg (author)

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

NATURVETENSKAP Fysik hsv//swe
NATURAL SCIENCES Physical Sciences hsv//eng
NATURVETENSKAP Fysik Atom- och molekylfysik och optik hsv//swe
NATURAL SCIENCES Physical Sciences Atom and Molecular Physics and Optics hsv//eng
DNA
Fluorescence microscopy
Nanofluidics
Single molecule
Emission enhancement
Emission intensity
Enhancement effects
Fluorescence enhancement
Optical phenomena
Quantitative fluorescence microscopy
Single DNA molecules
Molecules
Emission enhancement

Persson, Fredrik,1979Lund University,Lunds universitet,Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Physics,Departments at LTH,Faculty of Engineering, LTH,Technical University of Denmark(Swepub:lu)hist-fp0 (author)
Kristensen, A.Danmarks Tekniske Universitet,Technical University of Denmark (author)
Tegenfeldt, Jonas O.Lund University,Lunds universitet,Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)ftf-jte (author)
Göteborgs universitetInstitutionen för fysik (GU) (creator_code:org_t)

In:14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 20109781618390622

By the author/editor: Westerlund, Fred ...; Persson, Fredrik ...; Kristensen, A.; Tegenfeldt, Jona ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Physical Science ...; and Atom and Molecul ...

By the university: University of Gothenburg; Chalmers University of Technology; Lund University

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